Invitrogen

IFIT3 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

13个已发表图片

7 篇参考文献

查看其他16个IFIT3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

IFIT3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 21

IFIT3 Antibody (PA5-22230) in ICC/IF

Immunofluorescence analysis of Interferon-induced protein with tetratricopeptide repeats 3 was performed using 70% confluent log phase A549 treated with Human IFN Alpha (500 U/mL for 24 hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IFIT3 Polyclonal Antibody (Product # PA5-22230) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey a... View More

IFIT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IFIT3 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

IFIT3 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

IFIT3 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using IFIT3 Polyclonal Antibody (Product # PA5-22230), shows increased expression upon of IFIT3 in A549 cells upon human IFN Alpha A and IFN beta treatment., and in Daudi cells upon IFN Alpha treatment. {TM}

产品信息

PA5-22230

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 230 and 490 of IFIT3 (Uniprot ID#O14879)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.3 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_11153289

产品详细信息

Recommended positive controls: 293T.

Predicted reactivity: Chimpanzee (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exhibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Subviral Dense Bodies of Human Cytomegalovirus Induce an Antiviral Type I Interferon Response.

Cells

Penner I,Büscher N,Dejung M,Freiwald A,Butter F,Plachter B

Published figure using IFIT3 polyclonal antibody (Product # PA5-22230) in Western Blot

Tue Dec 13 00:00:00 EST 2022

CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states.

Nature neuroscience

Leng K,Rose IVL,Kim H,Xia W,Romero-Fernandez W,Rooney B,Koontz M,Li E,Ao Y,Wang S,Krawczyk M,Tcw J,Goate A,Zhang Y,Ullian EM,Sofroniew MV,Fancy SPJ,Schrag MS,Lippmann ES,Kampmann M

PA5-22230 was used in Immunocytochemistry-immunofluorescence to find that autocrine-paracrine IL-6 and interferon signaling downstream of canonical NF-κB activation drove two distinct inflammatory reactive signatures, one promoted by STAT3 and the other inhibited by STAT3.

Tue Nov 01 00:00:00 EDT 2022

Interferon-Induced Protein With Multiple Tetratricopeptide Repeats 3 Is Associated With Response to Chemotherapy and Recurrence but Not With Survival.

Pancreas

Popp MC,Klippstein M,Lohneis P,Kalinski T,Li J,Quaas A,Bludau M,Wang Z,Waldschmidt D,Kunzmann V,Damanakis A,Gebauer F,Zhao Y,Bruns CJ,Popp FC

Published figure using IFIT3 polyclonal antibody (Product # PA5-22230) in Immunohistochemistry

Tue Sep 28 00:00:00 EDT 2021

Inflammatory IFIT3 renders chemotherapy resistance by regulating post-translational modification of VDAC2 in pancreatic cancer.

Theranostics

Wang Z,Qin J,Zhao J,Li J,Li D,Popp M,Popp F,Alakus H,Kong B,Dong Q,Nelson PJ,Zhao Y,Bruns CJ

PA5-22230 was used in Immunoprecipitation to demonstrate a central mechanism by which IFIT3 expression can affect chemotherapy resistance in Pancreatic ductal adenocarcinoma (PDAC).

Mon May 03 00:00:00 EDT 2021

Inflammatory IFIT3 renders chemotherapy resistance by regulating post-translational modification of VDAC2 in pancreatic cancer.

Theranostics

Wang Z,Qin J,Zhao J,Li J,Li D,Popp M,Popp F,Alakus H,Kong B,Dong Q,Nelson PJ,Zhao Y,Bruns CJ

PA5-22230 was used in Immunoprecipitation to demonstrate a central mechanism by which IFIT3 expression can affect chemotherapy resistance in Pancreatic ductal adenocarcinoma (PDAC).

Mon May 03 00:00:00 EDT 2021

Inflammatory IFIT3 renders chemotherapy resistance by regulating post-translational modification of VDAC2 in pancreatic cancer.

Theranostics

Wang Z,Qin J,Zhao J,Li J,Li D,Popp M,Popp F,Alakus H,Kong B,Dong Q,Nelson PJ,Zhao Y,Bruns CJ

PA5-22230 was used in Immunoprecipitation to demonstrate a central mechanism by which IFIT3 expression can affect chemotherapy resistance in Pancreatic ductal adenocarcinoma (PDAC).

Mon May 03 00:00:00 EDT 2021

Inflammatory IFIT3 renders chemotherapy resistance by regulating post-translational modification of VDAC2 in pancreatic cancer.

Theranostics

Wang Z,Qin J,Zhao J,Li J,Li D,Popp M,Popp F,Alakus H,Kong B,Dong Q,Nelson PJ,Zhao Y,Bruns CJ

PA5-22230 was used in Immunoprecipitation to demonstrate a central mechanism by which IFIT3 expression can affect chemotherapy resistance in Pancreatic ductal adenocarcinoma (PDAC).

Mon May 03 00:00:00 EDT 2021

IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA.

Nucleic acids research

Fleith RC,Mears HV,Leong XY,Sanford TJ,Emmott E,Graham SC,Mansur DS,Sweeney TR

Published figure using IFIT3 polyclonal antibody (Product # PA5-22230) in Western Blot

Fri Jun 01 00:00:00 EDT 2018

生物信息学

蛋白别名: CIG49; GARG-49; Glucocorticoid-attenuated response gene 49 protein; IFI-60K; IFIT-3; IFIT-4; Interferon-induced 60 kDa protein; Interferon-induced protein with tetratricopeptide repeats 3; Interferon-induced protein with tetratricopeptide repeats 4; IRG2; ISG-60; P60; Retinoic acid-induced gene G protein; RP11-149I23.4

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基因别名: CIG-49; cig41; GARG-49; Garg49; Ifi49; IFI60; IFIT3; IFIT4; IRG2; Isg49; ISG60; P49; P60; RIG-G

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UniProt ID: (Human) O14879, (Mouse) Q64345

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Entrez Gene ID: (Human) 3437, (Mouse) 15959

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功能

molecular_function RNA binding protein binding identical protein binding

参与通路

immune system process cellular response to interferon-alpha cellular response to interferon-beta innate immune response defense response to virus negative regulation of cell proliferation response to virus negative regulation of apoptotic process type I interferon signaling pathway