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IKK epsilon Antibody (MA5-32807) in ICC/IF
Immunocytochemical analysis of IKK epsilon in SK-Br-3 cells using a IKK epsilon Monoclonal antibody (Product # MA5-32807) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
产品信息
MA5-32807
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JU06-72
抗原
Synthetic peptide within Human IKBKE aa 26-75
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2810083
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
IKBKE is an activator of nuclear factor-kappa-B. Over expression of IKBKE has been observed in breast cancer cells, and suppression of IKBKE induces cell death. NF-kappa-B is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappa-B is associated with Ikappa-B proteins in the cell cytoplasm, which inhibit NF-kappa-B activity. Ikappa-B is phosphorylated by Ikappa-B kinase (IKK) complex that contains IKK-a, IKK-b, and IKK-gamma. A novel molecule in the IKK complex was recently identified and designated IKK-iota/IKK-i. IKK epsilon is required for the activation of NF-kappa-B by PMA and T cell receptors but not by TNF-a and IL-1. IKK-iota/IKK-i message is expressed in a variety of tissues and is inducible by TNF-a, IL-1, and LPS.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IKK-i; IKK-related kinase epsilon; Inducible I kappa-B kinase; inducible IKappaB kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; RP11-534L20.1
基因别名: AW558201; IKBKE; IKK-E; IKK-i; IKKE; IKKepsilon; IKKI; KIAA0151
UniProt ID: (Human) Q14164, (Mouse) Q9R0T8
Entrez Gene ID: (Human) 9641, (Mouse) 56489
protein kinase activity
NF-kappaB-inducing kinase activity
protein binding
ATP binding
IkappaB kinase activity
ubiquitin protein ligase binding
K48-linked polyubiquitin binding
K63-linked polyubiquitin binding
nucleotide binding
protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein modifying enzyme
protein phosphorylation
immune response
I-kappaB phosphorylation
intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of type I interferon production
response to type I interferon
response to interferon-beta
TRIF-dependent toll-like receptor signaling pathway
NIK/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
protein homooligomerization
cellular response to virus
cellular response to DNA damage stimulus
phosphorylation
signal transduction by protein phosphorylation
