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IKZF4 Antibody (PA5-142535) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of IKZF4 in U2OS cells using IKZF4 Polyclonal Antibody (Product # PA5-142535). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
产品信息
PA5-142535
产品规格
种属反应
Human,
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptide with sequence C-RPTFIDRLANSLTKR (aa 309-323).
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
TBS, pH 7.3, with 0.5% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2933182
产品详细信息
Additional Information: This antibody is expected to recognise reported isoforms NP_071910.3; NP_001338019.1; NP_001338020.1; NP_001338021.1.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:2,000.
靶标信息
DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: IKAROS family zinc finger 4 (Eos); Ikaros family zinc finger protein 4; Zinc finger protein Eos; zinc finger protein, subfamily 1A, 4 (Eos); zinc finger transcription factor Eos
基因别名: A630026H08Rik; EOS; IKZF4; KIAA1782; Zfpn1a4; ZNFN1A4
UniProt ID: (Human) Q9H2S9, (Mouse) Q8C208
Entrez Gene ID: (Human) 64375, (Mouse) 22781
transcription factor activity, sequence-specific DNA binding
protein homodimerization activity
bHLH transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
protein heterodimerization activity
nucleic acid binding
DNA binding
protein binding
C2H2 zinc finger transcription factor
