Invitrogen

IL-1 beta Polyclonal Antibody, PeproTech®

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IL-1 beta Antibody (500-P51-1MG) in WB

Western Blot: To detect Murine IL-1beta by Western Blot analysis IL-1 beta Polyclonal Antibody (Product # 500-P51-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P51-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

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免疫组化 (IHC)

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酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

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阻断反应 (BLOCK)

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体外检测 (IV)

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免疫染色 (IS)

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产品规格

种属反应

Mouse

已发表种属

Bacteria, Human, Mouse

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Murine IL-1beta

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2930600

产品详细信息

AA Sequence of recombinant protein: MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQG EPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-1beta. Anti-Murine IL-1beta-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-1beta matrix.

Sandwich ELISA: To detect Murine IL-1beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-1beta (500-P51Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-1beta.

Western Blot: To detect Murine IL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Acute systemic inflammation exacerbates neuroinflammation in Alzheimer's disease: IL-1β drives amplified responses in primed astrocytes and neuronal network dysfunction.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Lopez-Rodriguez AB,Hennessy E,Murray CL,Nazmi A,Delaney HJ,Healy D,Fagan SG,Rooney M,Stewart E,Lewis A,de Barra N,Scarry P,Riggs-Miller L,Boche D,Cunningham MO,Cunningham C

Fri Oct 01 00:00:00 EDT 2021

Acute systemic inflammation exacerbates neuroinflammation in Alzheimer's disease: IL-1β drives amplified responses in primed astrocytes and neuronal network dysfunction.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Lopez-Rodriguez AB,Hennessy E,Murray CL,Nazmi A,Delaney HJ,Healy D,Fagan SG,Rooney M,Stewart E,Lewis A,de Barra N,Scarry P,Riggs-Miller L,Boche D,Cunningham MO,Cunningham C

Fri Oct 01 00:00:00 EDT 2021

Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine.

Communications biology

Klooster JPT,Bol-Schoenmakers M,van Summeren K,van Vliet ALW,de Haan CAM,van Kuppeveld FJM,Verkoeijen S,Pieters R

Thu May 27 00:00:00 EDT 2021

Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine.

Communications biology

Klooster JPT,Bol-Schoenmakers M,van Summeren K,van Vliet ALW,de Haan CAM,van Kuppeveld FJM,Verkoeijen S,Pieters R

Thu May 27 00:00:00 EDT 2021

Loss of endothelial glucocorticoid receptor accelerates diabetic nephropathy.

Nature communications

Srivastava SP,Zhou H,Setia O,Liu B,Kanasaki K,Koya D,Dardik A,Fernandez-Hernando C,Goodwin J

Thu Apr 22 00:00:00 EDT 2021

The Coagulation and Immune Systems Are Directly Linked through the Activation of Interleukin-1α by Thrombin.

Immunity

Burzynski LC,Humphry M,Pyrillou K,Wiggins KA,Chan JNE,Figg N,Kitt LL,Summers C,Tatham KC,Martin PB,Bennett MR,Clarke MCH

Tue Apr 16 00:00:00 EDT 2019

IL-1α cleavage by inflammatory caspases of the noncanonical inflammasome controls the senescence-associated secretory phenotype.

Aging cell

Wiggins KA,Parry AJ,Cassidy LD,Humphry M,Webster SJ,Goodall JC,Narita M,Clarke MCH

500-P51 was used in Western Blotting to investiate IL-1α upstream regulation.

Sat Jun 01 00:00:00 EDT 2019

IL-1α cleavage by inflammatory caspases of the noncanonical inflammasome controls the senescence-associated secretory phenotype.

Aging cell

Wiggins KA,Parry AJ,Cassidy LD,Humphry M,Webster SJ,Goodall JC,Narita M,Clarke MCH

500-P51 was used in Western Blotting to investiate IL-1α upstream regulation.

Sat Jun 01 00:00:00 EDT 2019

Dietary Toll-Like Receptor Stimulants Promote Hepatic Inflammation and Impair Reverse Cholesterol Transport in Mice via Macrophage-Dependent Interleukin-1 Production.

Frontiers in immunology

Faraj TA,Stover C,Erridge C

500-P51 was used in Western Blotting to demonstrate the link between consumption of processed food and the cardiovascular risks and how this can be combated by food microbiota.

Mon Oct 19 00:00:00 EDT 2020

Comparison of the virulence potential of Acinetobacter strains from clinical and environmental sources.

PloS one

Tayabali AF,Nguyen KC,Shwed PS,Crosthwait J,Coleman G,Seligy VL

Thu Sep 27 00:00:00 EDT 2012

Comparison of the virulence potential of Acinetobacter strains from clinical and environmental sources.

PloS one

Tayabali AF,Nguyen KC,Shwed PS,Crosthwait J,Coleman G,Seligy VL

Thu Sep 27 00:00:00 EDT 2012

Poor cerebral inflammatory response in eIF2B knock-in mice: implications for the aetiology of vanishing white matter disease.

PloS one

Cabilly Y,Barbi M,Geva M,Marom L,Chetrit D,Ehrlich M,Elroy-Stein O

Fri May 03 00:00:00 EDT 2013

Exacerbation of CNS inflammation and neurodegeneration by systemic LPS treatment is independent of circulating IL-1β and IL-6.

Journal of neuroinflammation

Murray CL,Skelly DT,Cunningham C

Tue May 17 00:00:00 EDT 2011

Systemic inflammation induces acute behavioral and cognitive changes and accelerates neurodegenerative disease.

Biological psychiatry

Cunningham C,Campion S,Lunnon K,Murray CL,Woods JF,Deacon RM,Rawlins JN,Perry VH

Sun Feb 15 00:00:00 EST 2009

Interleukin 15 expression in the CNS: blockade of its activity prevents glial activation after an inflammatory injury.

Glia

Gómez-Nicola D,Valle-Argos B,Pita-Thomas DW,Nieto-Sampedro M

Tue Apr 01 00:00:00 EDT 2008

Cortistatin, a new antiinflammatory peptide with therapeutic effect on lethal endotoxemia.

The Journal of experimental medicine

Gonzalez-Rey E,Chorny A,Robledo G,Delgado M

Mon Mar 20 00:00:00 EST 2006

生物信息学

蛋白别名: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta

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基因别名: Il-1b; IL-1beta; Il1b

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UniProt ID: (Mouse) P10749

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Entrez Gene ID: (Mouse) 16176

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功能

receptor binding cytokine activity interleukin-1 receptor binding protein domain specific binding interleukin superfamily

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