Invitrogen

IL-10 Monoclonal Antibody (12G8), Biotin

图: 1 / 2

IL-10 Antibody (M011B) in ELISA

Sandwich ELISA analysis of Rat anti-Human IL-10 biotinylated antibody (Product # M011B) was performed using the Thermo Scientific Human IL-10 Colorimetric ELISA kit (Product # EHIL10) by loading 50 µL per well of Human IL-10 recombinant protein (Product # SIL10) in triplicate at 600, 240, 96, 38.4, 15.36, and 0pg/mL across a 2 µg/mL Rat anti-Human IL-10 (Product # M010) pre-coated plate and incubated for 1.5 hours at room temperature. The plate was washed and incubated with 50 µL per well of Rat anti-Human IL-10 biotinylated antibody (Prod... View More

Product Image Gallery

产品信息

M011B

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1000

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

查看17篇已发表文章 17篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

12G8

抗原

Recombinant human IL-10 (COS cell-derived)

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein G

保存液

PBS with 4% BSA

内含物

proprietary preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_223553

产品详细信息

M011B targets IL-10 in ELISA and Western blot applications and shows reactivity with human samples.

The M011B immunogen is recombinant human IL-10 (COS cell-derived). The original parent clone is 12G8 and has been subcloned as 2553.2.4.

M011B detects IL-10 which has a predicted molecular weight of approximately 19 kDa.

The M011B IL10 antibody (biotinylated conjugate of clone 12G8) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M010 (clone 9D7). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M011B (clone 12G8) and antibody M010 (clone 9D7) have successfully been used in combination with recombinant IL10 protein SIL10 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

靶标信息

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Methods for in vitro generation of human type 1 regulatory T cells.

Methods in molecular biology (Clifton, N.J.)

Gregori S,Roncarolo MG,Bacchetta R

M011B was used in ELISA to study cytokine production profile of Tr1 cell lines

Wed Feb 02 00:00:00 EST 2011

Molecular and functional characterization of allogantigen-specific anergic T cells suitable for cell therapy.

Haematologica

Bacchetta R,Gregori S,Serafini G,Sartirana C,Schulz U,Zino E,Tomiuk S,Jansen U,Ponzoni M,Paties CT,Fleischhauer K,Roncarolo MG

M011B was used in ELISA to characterize allogantigen-specific anergic T cells

Wed Dec 01 00:00:00 EST 2010

Hepatitis C virus modulates human monocyte-derived dendritic cells.

Journal of viral hepatitis

Eksioglu EA,Bess JR,Zhu H,Xu Y,Dong HJ,Elyar J,Nelson DR,Liu C

M011B was used in ELISA to study the effect of hepatitis C virus on human monocyte-derived dendritic cells

Mon Nov 01 00:00:00 EDT 2010

Type 1 regulatory T cells are associated with persistent split erythroid/lymphoid chimerism after allogeneic hematopoietic stem cell transplantation for thalassemia.

Haematologica

Serafini G,Andreani M,Testi M,Battarra M,Bontadini A,Biral E,Fleischhauer K,Marktel S,Lucarelli G,Roncarolo MG,Bacchetta R

M011B was used in ELISA to investigate the role of type 1 regulatory T cells during persistent split erythroid/lymphoid chimerism after allogeneic hematopoietic stem cell transplantation for thalassemia

Thu Oct 01 00:00:00 EDT 2009

Anti-ribosomal phosphoprotein autoantibody triggers interleukin-10 overproduction via phosphatidylinositol 3-kinase-dependent signalling pathways in lipopolysaccharide-activated macrophages.

Immunology

Lee TP,Leu SJ,Huang JC,Song YC,Jhou RS,Tang SJ,Sun KH

M011B was used in ELISA to investigate the mechanism of interleukin 10 overproduction induced by anti-ribosomal phosphoprotein autoantibody in lipopolysaccharide-activated macrophages

Fri May 01 00:00:00 EDT 2009

Hepatitis C virus-specific reactivity of CD4+-lymphocytes in children born from HCV-infected women.

Journal of hepatology

Della Bella S,Riva A,Tanzi E,Nicola S,Amendola A,Vecchi L,Nebbia G,Longhi R,Zanetti AR,Villa ML

M011B was used in ELISA to investigate the reactivity of CD4+ lymphocytes specific to hepatitis C virus in children born from HCV-infected women

Thu Sep 01 00:00:00 EDT 2005

Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.

Rheumatology (Oxford, England)

Schotte H,Schlüter B,Willeke P,Mickholz E,Schorat MA,Domschke W,Gaubitz M

M011B was used in ELISA to study the effect of etanercept treatment on proinflammatory cytokine-secreting peripheral blood mononuclear cells in rheumatoid arthritic patients

Sun Aug 01 00:00:00 EDT 2004

Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients.

Rheumatology international

Solomon S,Masilamani M,Mohanty S,Schwab JE,Boneberg EM,Illges H

M011B was used in ELISA to investigate the generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients

Mon Mar 01 00:00:00 EST 2004

Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients.

Journal of hepatology

Loffreda S,Muratori P,Muratori L,Mele L,Bianchi FB,Lenzi M

M011B was used in ELISA to investigate the augmented production of Th1-type cytokines in HCV-infected cryoglobulinemic patients

Sat Feb 01 00:00:00 EST 2003

Altered maturation of peripheral blood dendritic cells in patients with breast cancer.

British journal of cancer

Della Bella S,Gennaro M,Vaccari M,Ferraris C,Nicola S,Riva A,Clerici M,Greco M,Villa ML

M011B was used in ELISA to characterize the peripheral blood dendritic cell maturation in breast cancer patients

Mon Oct 20 00:00:00 EDT 2003

Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.

Transplantation proceedings

Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M

M011B was used in ELISA to investigate the effect of immunosuppressive regimens on cytokine expression in lung transplanted patients

Sun Jun 01 00:00:00 EDT 2003

The neuropeptide bombesin improves IgA-mediated mucosal immunity with preservation of gut interleukin-4 in total parenteral nutrition-fed mice.

Surgery

Zarzaur BL,Wu Y,Fukatsu K,Johnson CD,Kudsk KA

M011B was used in ELISA to study the interleukin levels in the mice with various diets

Tue Jan 01 00:00:00 EST 2002

Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent.

Infection and immunity

McDowell MA,Marovich M,Lira R,Braun M,Sacks D

M011B was used in ELISA to investigate the mechanism for the effect of Leishmania on interleukin 12 p70 secretion

Thu Aug 01 00:00:00 EDT 2002

Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.

Experimental lung research

Suwa T,Hogg JC,Vincent R,Mukae H,Fujii T,van Eeden SF

M011B was used in ELISA to test if factors released from human alveolar macrophages exposed to air pollution particles accelerate the maturation of granulocyte precursors

Tue Jul 09 00:00:00 EDT 2002

Mistletoe lectin-1 increases tumor necrosis factor-alpha release in lipopolysaccharide-stimulated whole blood via inhibition of interleukin-10 production.

The Journal of pharmacology and experimental therapeutics

Boneberg EM,Hartung T

M011B was used in flow cytometry to investigate the mechanims for the effect of mistletoe lectin-1 on tumor necrosis factor-alpha release

Sat Sep 01 00:00:00 EDT 2001

Autoregulation of human monocyte-derived dendritic cell maturation and IL-12 production by cyclooxygenase-2-mediated prostanoid production.

Journal of immunology (Baltimore, Md. : 1950)

Whittaker DS,Bahjat KS,Moldawer LL,Clare-Salzler MJ

M011B was used in ELISA to study the mechanism for the effect of prostaglandins on the maturation and function of monocyte-derived dendritic cells

Sun Oct 15 00:00:00 EDT 2000

Nitric oxide in Tanzanian children with malaria: inverse relationship between malaria severity and nitric oxide production/nitric oxide synthase type 2 expression.

The Journal of experimental medicine

Anstey NM,Weinberg JB,Hassanali MY,Mwaikambo ED,Manyenga D,Misukonis MA,Arnelle DR,Hollis D,McDonald MI,Granger DL

M011B was used in ELISA to measure the interleukin-10 and interleukin-4 level in Tanzanian children with malaria

Thu Aug 01 00:00:00 EDT 1996

Contribution of IL-1, CD14, and CD13 in the increased IL-6 production induced by in vitro monocyte-synoviocyte interactions.

Journal of immunology (Baltimore, Md. : 1950)

Chomarat P,Rissoan MC,Pin JJ,Banchereau J,Miossec P

M011B was used in ELISA to investigate the effect of interleukin 1, CD14, and CD13 on the expression of interleukin 6 produced by monocytes and synoviocytes in vitro

Sun Oct 01 00:00:00 EDT 1995

生物信息学

蛋白别名: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

查看更多收起

基因别名: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

查看更多收起

UniProt ID: (Human) P22301

查看更多收起

Entrez Gene ID: (Human) 3586

查看更多收起

功能

cytokine activity interleukin-10 receptor binding protein binding growth factor activity

参与通路