Invitrogen

IL-10 Monoclonal Antibody (945A5D11)

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IL-10 Antibody (AHC8102) in ELISA

The Journal of experimental medicine 1999 - Figure 3 CT treatment selectively suppressed IL-12 p70 and TNF-alpha, but not IL-10, TGF-beta 1 , or IL-6. Human monocytes (2 x 10 6 cell/mL) were stimulated with SAC (0.01%) and IFN-gamma (100 ng/mL) in the presence or absence of CT (10 ng/mL). Cytokine production was measured by ELISA 24 h after stimulation. Data are mean values +- SD of four separate experiments using four different donors. ** P <... View More

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产品信息

AHC8102

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

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酶联免疫吸附实验 (ELISA)

Assay-dependent

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产品规格

种属反应

Human

已发表种属

Human, Non-human primate, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

945A5D11

抗原

Recombinant human IL-10.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.5mg/mL sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2536291

产品详细信息

This product is recommended as a capture antibody in Sandwich ELISA applications.

靶标信息

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Oral biopsies from patients with orofacial granulomatosis with histology resembling Crohn's disease have a prominent Th1 environment.

Inflammatory bowel diseases

Freysdottir J,Zhang S,Tilakaratne WM,Fortune F

AHC8102 was used in immunohistochemistry - paraffin section to discuss if patients with Crohn's disease and orofacial granulomatosis should be considered as a unique subset

Sun Apr 01 00:00:00 EDT 2007

The effect of aging and inflammation on heat shock protein 27 in human monocytes and lymphocytes.

Experimental gerontology

Njemini R,Lambert M,Demanet C,Mets T

AHC8102 was used in ELISA to examine the effect of age and inflammation on the induction of Hsp27 in human peripheral blood mononuclear cells

Wed Mar 01 00:00:00 EST 2006

Insulin-like growth factor-I stimulates IL-10 production in human T cells.

Journal of leukocyte biology

Kooijman R,Coppens A

AHC8102 was used in ELISA to explore the immunomodulatory effects of insulin-like growth factor through regulation of cytokine production

Fri Oct 01 00:00:00 EDT 2004

Selective suppression of IL-12 production by chemoattractants.

Journal of immunology (Baltimore, Md. : 1950)

Braun MC,Lahey E,Kelsall BL

AHC8102 was used in ELISA to determine the ability of chemoattractants to affect IL-2 production by human monocytes and dendritic cells.

Wed Mar 15 00:00:00 EST 2000

Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.

Journal of immunology (Baltimore, Md. : 1950)

Farah IO,Mola PW,Kariuki TM,Nyindo M,Blanton RE,King CL

AHC8102 was used in ELISA to test if repeated exposure to Schistosoma mansoni stimulates hepatic fibrosis in olive baboons.

Mon May 15 00:00:00 EDT 2000

Activation of toll-like receptor 2 on human dendritic cells triggers induction of IL-12, but not IL-10.

Journal of immunology (Baltimore, Md. : 1950)

Thoma-Uszynski S,Kiertscher SM,Ochoa MT,Bouis DA,Norgard MV,Miyake K,Godowski PJ,Roth MD,Modlin RL

AHC8102 was used in ELISA to compare the expression and response of TLRs on monocytes and dendritic cells.

Sun Oct 01 00:00:00 EDT 2000

Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.

The Journal of experimental medicine

Braun MC,He J,Wu CY,Kelsall BL

AHC8102 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.

Mon Feb 01 00:00:00 EST 1999

Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.

The Journal of experimental medicine

Braun MC,He J,Wu CY,Kelsall BL

AHC8102 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.

Mon Feb 01 00:00:00 EST 1999

Protective immunity using recombinant human IL-12 and alum as adjuvants in a primate model of cutaneous leishmaniasis.

Journal of immunology (Baltimore, Md. : 1950)

Kenney RT,Sacks DL,Sypek JP,Vilela L,Gam AA,Evans-Davis K

AHC8102 was used in ELISA to use rhIL-12 and alum as adjuvants in primate model of cutaneous leishmaniasis to test if the killed pathogen is a sufficient to generate protective antibodies.

Fri Oct 15 00:00:00 EDT 1999

Cytokine control of the granulomatous response in Schistosoma mansoni-infected baboons: role of exposure and treatment.

Infection and immunity

Mola PW,Farah IO,Kariuki TM,Nyindo M,Blanton RE,King CL

AHC8102 was used in ELISA to test if variations in exposure and treatment contribute to the heterogeneity in immunity and granuloma-induced pathology in schistosomiasis.

Wed Dec 01 00:00:00 EST 1999

生物信息学

蛋白别名: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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基因别名: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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功能

cytokine activity interleukin-10 receptor binding protein binding growth factor activity

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