Invitrogen

IL-10 Monoclonal Antibody (JES3-9D7)

产品信息

AHC0102

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

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流式细胞分析 (Flow)

Assay-dependent

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酶联免疫吸附实验 (ELISA)

Assay-dependent

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中和实验 (Neu)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Mouse, Non-human primate, Rat, Rhesus monkey

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

JES3-9D7

抗原

Recombinant human Interleukin 10.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

-20°C

RRID

AB_2536243

靶标信息

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Sevoflurane exerts protective effects on liver ischemia/reperfusion injury by regulating NFKB3 expression via miR-9-5p.

Experimental and therapeutic medicine

Liao X,Zhou S,Zong J,Wang Z

AHC0103 was used in an ELISA assay to study the protective effects of sevoflurane on hepatic ischemia/reperfusion liver injury.

Mon Apr 01 00:00:00 EDT 2019

Optimization of mass cytometry sample cryopreservation after staining.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Sumatoh HR,Teng KW,Cheng Y,Newell EW

AHC0102 was used in flow cytometry to demonstrate that freezing already-stained samples suspended in 10% DMSO in FBS is practical and efficient way to preserve already-stained samples for mass cytometry assessment

Sun Jan 01 00:00:00 EST 2017

Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.

PLoS pathogens

Miles B,Miller SM,Folkvord JM,Levy DN,Rakasz EG,Skinner PJ,Connick E

AHC0102 was used in flow cytometry to identify and characterize CXCR5hiCD44hiCD8 T cells in HIV-infected individuals

Sat Oct 01 00:00:00 EDT 2016

Mucosal Inducible NO Synthase-Producing IgA+ Plasma Cells in Helicobacter pylori-Infected Patients.

Journal of immunology (Baltimore, Md. : 1950)

Neumann L,Mueller M,Moos V,Heller F,Meyer TF,Loddenkemper C,Bojarski C,Fehlings M,Doerner T,Allers K,Aebischer T,Ignatius R,Schneider T

AHC0102 was used in flow cytometry to report that IgA-producing plasma cells are a major source of inducible NO synthase in Helicobacter pylori-infected patients

Thu Sep 01 00:00:00 EDT 2016

Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques.

Infection and immunity

Phuah J,Wong EA,Gideon HP,Maiello P,Coleman MT,Hendricks MR,Ruden R,Cirrincione LR,Chan J,Lin PL,Flynn JL

AHC0102 was used in flow cytometry to report that B cells modulate the local granulomatous response in Mycobacterium tuberculosis-infected macaques during acute infection

Sun May 01 00:00:00 EDT 2016

CCR10/CCL27 crosstalk contributes to failure of proteasome-inhibitors in multiple myeloma.

Oncotarget

Thangavadivel S,Zelle-Rieser C,Olivier A,Postert B,Untergasser G,Kern J,Brunner A,Gunsilius E,Biedermann R,Hajek R,Pour L,Willenbacher W,Greil R,Jöhrer K

AHC0102 was used in an ELISA assay to study bone marrow derived chemokines of naive and therapy refractory myeloma patients.

Tue Nov 29 00:00:00 EST 2016

CD4+ T-cell responses among adults and young children in response to Streptococcus pneumoniae and Haemophilus influenzae vaccine candidate protein antigens.

Vaccine

Sharma SK,Roumanes D,Almudevar A,Mosmann TR,Pichichero ME

AHC0102 was used in flow cytometry to determine cytokine profiles of CD4(+) T-helper (h) cells in adults and young children vaccinated with the antigens PspA, PcpA, PhtD, PhtE, Ply, LytB of Streptococcus pneumonia and protein D and OMP26 of non-typeable Haemophilus influenza.

Wed Jun 26 00:00:00 EDT 2013

Type III interferons, IL-28 and IL-29, are increased in chronic HCV infection and induce myeloid dendritic cell-mediated FoxP3+ regulatory T cells.

PloS one

Dolganiuc A,Kodys K,Marshall C,Saha B,Zhang S,Bala S,Szabo G

AHC0102 was used in blocking assay to study if IFN-λ has immunomodulatory effects in HCV- infected individuals.

Tue Apr 02 00:00:00 EDT 2013

Systemic and compartmentalised immune responses in a Leishmania braziliensis-macaque model of self-healing cutaneous leishmaniasis.

Veterinary immunology and immunopathology

de-Campos SN,Souza-Lemos C,Teva A,Porrozzi R,Grimaldi G

AHC0102 was used in flow cytometry to characterizes the systemic and local cell-mediated immune responses control granuloma growth in Leishmania braziliensis-infected maxaques

Wed Sep 15 00:00:00 EDT 2010

Application of six-color flow cytometry for the assessment of dendritic cell responses in whole blood assays.

Journal of immunological methods

Della Bella S,Giannelli S,Taddeo A,Presicce P,Villa ML

AHC0102 was used in flow cytometry to describe a 6-color assay to analyze peripheral blood dendritic cell responses upon whole blood stimulation.

Wed Dec 31 00:00:00 EST 2008

TGF-beta in intestinal lymphoid organs contributes to the death of armed effector CD8 T cells and is associated with the absence of virus containment in rhesus macaques infected with the simian immunodeficiency virus.

Cell death and differentiation

Cumont MC,Monceaux V,Viollet L,Lay S,Parker R,Hurtrel B,Estaquier J

AHC0102 was used in immunohistochemistry to examine links between SIV viral reservoir sites, CD8+ T-cell activation/death and outcome

Mon Oct 01 00:00:00 EDT 2007

Expression and functional characterization of FOXP3+ CD4+ regulatory T cells in ulcerative colitis.

Inflammatory bowel diseases

Yu QT,Saruta M,Avanesyan A,Fleshner PR,Banham AH,Papadakis KA

AHC0102 was used in blocking or activating experiment to determine the presence and functional characteristics of T regulatory cells in colonic lymphoid tissues in patients with ulcerative colitis

Thu Feb 01 00:00:00 EST 2007

Characterization of Foxp3+CD4+CD25+ and IL-10-secreting CD4+CD25+ T cells during cure of colitis.

Journal of immunology (Baltimore, Md. : 1950)

Uhlig HH,Coombes J,Mottet C,Izcue A,Thompson C,Fanger A,Tannapfel A,Fontenot JD,Ramsdell F,Powrie F

AHC0102 was used in immunohistochemistry - paraffin section to determine the in vivo distribution of Foxp3+CD4+CD25+ cells in mice that have recovered from colitis

Wed Nov 01 00:00:00 EST 2006

IL-10 polymorphism and cell-mediated immune response to Chlamydia trachomatis.

Genes and immunity

Ohman H,Tiitinen A,Halttunen M,Birkelund S,Christiansen G,Koskela P,Lehtinen M,Paavonen J,Surcel HM

AHC0102 was used in blocking or activating experiment to assess the relationship between interleukin-10 promoter -1082 polymorphism and cell-mediated immune response during Chlamydia trachomatis infection

Sat Apr 01 00:00:00 EST 2006

Transforming growth factor beta 1 production by CD4+ CD25+ regulatory T cells in peripheral blood mononuclear cells from healthy subjects stimulated with Leishmania guyanensis.

Infection and immunity

Kariminia A,Bourreau E,Pascalis H,Couppié P,Sainte-Marie D,Tacchini-Cottier F,Launois P

AHC0102 was used in blocking or activating experiment to show that TGF-beta1 is involved in the suppressive activity of Leishmania guyanensis-stimulated CD4+ CD25+ T cells from healthy controls

Thu Sep 01 00:00:00 EDT 2005

CD4+ PD-1+ T cells accumulate as unique anergic cells in rheumatoid arthritis synovial fluid.

The Journal of rheumatology

Hatachi S,Iwai Y,Kawano S,Morinobu S,Kobayashi M,Koshiba M,Saura R,Kurosaka M,Honjo T,Kumagai S

AHC0102 was used in flow cytometry to investigate the involvement of the PD-1 system in rheumatoid arthritis

Tue Jul 01 00:00:00 EDT 2003

Escaping high viral load exhaustion: CD8 cells with altered tetramer binding in chronic hepatitis B virus infection.

The Journal of experimental medicine

Reignat S,Webster GJ,Brown D,Ogg GS,King A,Seneviratne SL,Dusheiko G,Williams R,Maini MK,Bertoletti A

AHC0102 was used in flow cytometry to characterize CD8 cells present in chronic hepatitis B virus.

Mon May 06 00:00:00 EDT 2002

Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines.

Journal of immunology (Baltimore, Md. : 1950)

Cousins DJ,Lee TH,Staynov DZ

AHC0102 was used in flow cytometry to report an in vitro differentiation assay in which human naive CD4(+) cells are driven toward either the Th1 or Th2 phenotype and study them.

Sun Sep 01 00:00:00 EDT 2002

Flow cytometric analysis of gut mucosal lymphocytes supports an impaired Th1 cytokine profile in spondyloarthropathy.

Annals of the rheumatic diseases

Van Damme N,De Vos M,Baeten D,Demetter P,Mielants H,Verbruggen G,Cuvelier C,Veys EM,De Keyser F

AHC0102 was used in flow cytometry to measure cytokine production by intestinal T cells isolated from patients with spondyloarthropathy.

Tue May 01 00:00:00 EDT 2001

Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype).

Journal of medical virology

Hutchinson KL,Villinger F,Miranda ME,Ksiazek TG,Peters CJ,Rollin PE

Thu Nov 01 00:00:00 EST 2001

Cytokine responses to treponema pectinovorum and treponema denticola in human gingival fibroblasts.

Infection and immunity

Nixon CS,Steffen MJ,Ebersole JL

Fri Sep 01 00:00:00 EDT 2000

A soluble factor produced by lamina propria mononuclear cells is required for TNF-alpha enhancement of IFN-gamma production by T cells.

Journal of immunology (Baltimore, Md. : 1950)

Prehn JL,Landers CJ,Targan SR

AHC0102 was used in ELISA to elucidate the mechanism of TNF-alpha-augmented mucosal T cell IFN-gamma production.

Fri Oct 15 00:00:00 EDT 1999

Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies.

Molecular pharmacology

Koshiba M,Rosin DL,Hayashi N,Linden J,Sitkovsky MV

AHC0102 was used in flow cytometry to elucidate the role of A2ARs in the regulation of immune response.

Mon Mar 01 00:00:00 EST 1999

Transient monocyte release of interleukin-10 in response to traumatic brain injury.

Shock (Augusta, Ga.)

Shimonkevitz R,Bar-Or D,Harris L,Dole K,McLaughlin L,Yukl R

AHC0102 was used in flow cytometry to measure cytokines after head injury.

Thu Jul 01 00:00:00 EDT 1999

Cross-linking of Fc(gamma)-receptor on monocytes inhibits hepatitis C virus-specific cytotoxic T-lymphocyte induction in vitro.

Immunology

Kanto T,Hayashi N,Takehara T,Katayama K,Ito A,Mochizuki K,Kuzushita N,Tatsumi T,Sasaki Y,Kasahara A,Hori M

AHC0102 was used in blocking assay to determine the roles of immune complexes in HCV-specific cytotoxic T lymphocyte induction.

Sat Aug 01 00:00:00 EDT 1998

Immune complexes are potent inhibitors of interleukin-12 secretion by human monocytes.

European journal of immunology

Berger S,Chandra R,Balló H,Hildenbrand R,Stutte HJ

AHC0102 was used in blocking assay to examine the effect of immune complexes on interleukin-12 secretion by human monocytes.

Sat Nov 01 00:00:00 EST 1997

Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.

Immunological reviews

Abrams JS,Roncarolo MG,Yssel H,Andersson U,Gleich GJ,Silver JE

Mon Jun 01 00:00:00 EDT 1992

Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.

Immunological reviews

Abrams JS,Roncarolo MG,Yssel H,Andersson U,Gleich GJ,Silver JE

Mon Jun 01 00:00:00 EDT 1992

Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.

Immunological reviews

Abrams JS,Roncarolo MG,Yssel H,Andersson U,Gleich GJ,Silver JE

Mon Jun 01 00:00:00 EDT 1992

生物信息学

蛋白别名: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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基因别名: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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功能

cytokine activity interleukin-10 receptor binding protein binding growth factor activity

参与通路