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Invitrogen

IL-13 Monoclonal Antibody (eBio13A), APC-eFluor™ 780, eBioscience™

25个已发表图片

21 篇参考文献

查看其他64个IL-13抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (1)
已发表图片 (25)

Application

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IL-13 Antibody (47-7133-82) in Flow

BALB/c splenocytes were stimulated for 3 days with plate-bound Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82), soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82), Mouse IL-2 Recombinant Protein (Product # 14-8021-64), and Mouse IL-4 Recombinant Protein (Product # 14-8041-80). Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) for 5 hours. Following restimulation, cells were fixed and permeabilized (Product # 88-8824-00) and then stai... View More

IL-13 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

IL-13 Antibody in Flow Cytometry, ELISA (Flow, ELISA)

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产品信息

47-7133-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看19篇已发表文章 19篇已发表文章

酶联免疫吸附实验 (ELISA)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio13A

偶联物

APC-eFluor™ 780

激发/发射光谱

756/785 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2716964

产品详细信息

Description: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing. In cancer models it has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)

Applications Reported: This eBio13A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio13A antibody has been tested by intracellular staining and flow cytometric analysis of Th2-polarized mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

靶标信息

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder

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Product References

Celastrol alleviates atopic dermatitis by regulating Ezrin-mediated mitochondrial fission and fusion.

Journal of cellular and molecular medicine

Wang D,Jin S,Liu H,Song X,Jin H,Song Y,Zhao H,Li L,Yan G

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Mon Jul 01 00:00:00 UTC 2024

Group 2 innate lymphoid cells suppress neuroinflammation and brain injury following intracerebral hemorrhage.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Liu M,Wang D,Xu L,Pan Y,Huang H,Li M,Liu Q

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Fri Mar 01 00:00:00 UTC 2024

TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation.

The Journal of experimental medicine

Schmitt P,Duval A,Camus M,Lefrançais E,Roga S,Dedieu C,Ortega N,Bellard E,Mirey E,Mouton-Barbosa E,Burlet-Schiltz O,Gonzalez-de-Peredo A,Cayrol C,Girard JP

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Mon Jun 03 00:00:00 UTC 2024

Self-amplifying mRNA seasonal influenza vaccines elicit mouse neutralizing antibody and cell-mediated immunity and protect ferrets.

NPJ vaccines

Cheung M,Chang C,Rathnasinghe R,Rossignol E,Zhang Y,Ferrari A,Patel H,Huang Y,Sanchez Guillen M,Scalzo T,Lee C,Otten GR,Settembre EC,Music N,Palladino G,Wen Y

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Wed Oct 04 00:00:00 UTC 2023

Non-glycosylated G protein with CpG ODN provides robust protection against respiratory syncytial virus without inducing eosinophilia.

Frontiers in immunology

Kawahara E,Shibata T,Hirai T,Yoshioka Y

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Fri Jan 05 00:00:00 UTC 2024

Solar ultraviolet B radiation promotes α-MSH secretion to attenuate the function of ILC2s via the pituitary-lung axis.

Nature communications

Huang Y,Zhu L,Cheng S,Dai R,Huang C,Song Y,Peng B,Li X,Wen J,Gong Y,Hu Y,Qian L,Zhu L,Zhang F,Yu L,Yi C,Gu W,Ling Z,Ma L,Tang W,Peng L,Shi G,Zhang Y,Sun B

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Sep 12 00:00:00 UTC 2023

Monitoring Group 2 Innate Lymphoid Cell Biology in Models of Lung Inflammation.

Bio-protocol

Badrani JH,Strohm AN,Haung YA,Doherty TA

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Thu Jul 20 00:00:00 UTC 2023

Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice.

Nature communications

Cheong LY,Wang B,Wang Q,Jin L,Kwok KHM,Wu X,Shu L,Lin H,Chung SK,Cheng KKY,Hoo RLC,Xu A

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Fri Mar 03 00:00:00 UTC 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Sat Apr 01 00:00:00 UTC 2023

Mitochondrial transcription factor A in RORγt⁺ lymphocytes regulate small intestine homeostasis and metabolism.

Nature communications

Fu Z,Dean JW,Xiong L,Dougherty MW,Oliff KN,Chen ZE,Jobin C,Garrett TJ,Zhou L

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Thu Jul 22 00:00:00 UTC 2021

Synergistic effect of non-neutralizing antibodies and interferon-γ for cross-protection against influenza.

iScience

Shibuya M,Tamiya S,Kawai A,Hirai T,Cragg MS,Yoshioka Y

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in ELISA

Fri Oct 22 00:00:00 UTC 2021

Synergistic effect of non-neutralizing antibodies and interferon-γ for cross-protection against influenza.

iScience

Shibuya M,Tamiya S,Kawai A,Hirai T,Cragg MS,Yoshioka Y

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in ELISA

Fri Oct 22 00:00:00 UTC 2021

Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

Frontiers in immunology

Liu J,Liu Y,Panda S,Liu A,Lei J,Burd I

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Fri Dec 17 00:00:00 UTC 2021

Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL-33/ST2 Signaling.

Frontiers in immunology

Deng F,Hu JJ,Yang X,Sun QS,Lin ZB,Zhao BC,Yao ZW,Luo SD,Chen ZL,Liu Y,Yan ZZ,Li C,Liu WF,Liu KX

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Dec 21 00:00:00 UTC 2021

Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL-33/ST2 Signaling.

Frontiers in immunology

Deng F,Hu JJ,Yang X,Sun QS,Lin ZB,Zhao BC,Yao ZW,Luo SD,Chen ZL,Liu Y,Yan ZZ,Li C,Liu WF,Liu KX

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Dec 21 00:00:00 UTC 2021

6-Phosphogluconate dehydrogenase (6PGD), a key checkpoint in reprogramming of regulatory T cells metabolism and function.

eLife

Daneshmandi S,Cassel T,Higashi RM,Fan TW,Seth P

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Thu Oct 28 00:00:00 UTC 2021

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Nov 23 00:00:00 UTC 2021

A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma.

Nature immunology

Harb H,Stephen-Victor E,Crestani E,Benamar M,Massoud A,Cui Y,Charbonnier LM,Arbag S,Baris S,Cunnigham A,Leyva-Castillo JM,Geha RS,Mousavi AJ,Guennewig B,Schmitz-Abe K,Sioutas C,Phipatanakul W,Chatila TA

47-7133 was used in Flow cytometry/Cell sorting to find that interleukin-6- and STAT3 transcription factor-dependent upregulation of Notch4 receptor on lung tissue regulatory T (Treg) cells is necessary for allergens and particulate matter pollutants to promote airway inflammation.

Sun Nov 01 00:00:00 UTC 2020

A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma.

Nature immunology

Sun Nov 01 00:00:00 UTC 2020

A helminth-derived suppressor of ST2 blocks allergic responses.

eLife

Vacca F,Chauché C,Jamwal A,Hinchy EC,Heieis G,Webster H,Ogunkanbi A,Sekne Z,Gregory WF,Wear M,Perona-Wright G,Higgins MK,Nys JA,Cohen ES,McSorley HJ

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Mon May 18 00:00:00 UTC 2020

TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity.

Cell reports

Hurrell BP,Galle-Treger L,Jahani PS,Howard E,Helou DG,Banie H,Soroosh P,Akbari O

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Dec 24 00:00:00 UTC 2019

Nematode-Infected Mice Acquire Resistance to Subsequent Infection With Unrelated Nematode by Inducing Highly Responsive Group 2 Innate Lymphoid Cells in the Lung.

Frontiers in immunology

Yasuda K,Adachi T,Koida A,Nakanishi K

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Mon Sep 30 00:00:00 UTC 2019

生物信息学

蛋白别名: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600

基因别名: Il-13; Il13

UniProt ID: (Mouse) P20109

Entrez Gene ID: (Mouse) 16163

功能

cytokine activity cytokine receptor binding interleukin-13 receptor binding

参与通路

IL-13 Monoclonal Antibody (eBio13A), APC-eFluor™ 780, eBioscience™

IL-13 Antibody (47-7133-82) in Flow

产品信息

47-7133-82

免疫组化 (IHC)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Your items have has been added!

IL-13 Monoclonal Antibody (eBio13A), APC-eFluor™ 780, eBioscience™

IL-13 Antibody (47-7133-82) in Flow

产品信息

47-7133-82

免疫组化 (IHC)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID