Target of Interest
Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
图: 1 / 1
IL-18 Antibody (PA5-46961) in ICC/IF
Immunocytochemistry analysis of IL-18 in immersion fixed porcine peripheral blood mononuclear cells (PBMCs). Samples were incubated in IL-18 polyclonal antibody (Product # PA5-46961) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
产品信息
PA5-46961
产品规格
种属反应
Pig
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived recombinant porcine IL-18/IL-1F4
偶联物
Unconjugated
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2610330
产品详细信息
In direct ELISAs, approximately 30% cross-reactivity with recombinant human IL-18 is observed and 10% cross-reactivity with recombinant rat IL-18 and recombinant mouse IL-18 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
靶标信息
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: cytokine; IFN-gamma-inducing factor; il 18; IL-1 gamma; IL-18; ILN; Interferon gamma-inducing factor; Interleukin; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; Interleukin-18; Interleukin18; MGC12320
基因别名: IGIF; IL-18; IL18
UniProt ID: (Pig) O19073
Entrez Gene ID: (Pig) 397057
MAPK cascade
angiogenesis
inflammatory response
cell proliferation
positive regulation of cell proliferation
extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of macrophage derived foam cell differentiation
positive regulation of phosphatidylinositol 3-kinase signaling
natural killer cell activation
lipopolysaccharide-mediated signaling pathway
activation of protein kinase B activity
interferon-gamma production
interleukin-6 production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of natural killer cell proliferation
interleukin-18-mediated signaling pathway
T-helper 1 cell cytokine production
positive regulation of activated T cell proliferation
neutrophil activation
natural killer cell mediated cytotoxicity
positive regulation of tyrosine phosphorylation of STAT protein
cholesterol homeostasis
positive regulation of T-helper 2 cell differentiation
negative regulation of myoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
positive regulation of NF-kappaB transcription factor activity
positive regulation of NK T cell proliferation
positive regulation of protein kinase B signaling
triglyceride homeostasis
cellular response to organic cyclic compound
positive regulation of cold-induced thermogenesis
positive regulation of NIK/NF-kappaB signaling
positive regulation of T-helper 1 cell cytokine production
