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IL-5 Antibody (500-P55-1MG) in WB
Western Blot: To detect Murine IL-5 by Western Blot analysis IL-5 Polyclonal Antibody (Product # 500-P55-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P55-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine IL-5
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930624
产品详细信息
AA Sequence of recombinant protein: MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-5. Anti-Murine IL-5-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-5 matrix.
Sandwich ELISA: To detect Murine IL-5 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-5 (500-P55Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-5.
Western Blot: To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF
基因别名: Il-5; Il5
UniProt ID: (Mouse) P04401
Entrez Gene ID: (Mouse) 16191
immune response
positive regulation of cell proliferation
cytokine-mediated signaling pathway
positive regulation of B cell proliferation
positive regulation of eosinophil differentiation
positive regulation of transcription, DNA-templated
positive regulation of JAK-STAT cascade
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of immunoglobulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of podosome assembly
