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IL-5 Antibody (M550B) in ELISA
Sandwich ELISA analysis of human IL-5 was performed by coating wells of a 96-well plate with 110 µL of a human IL-5 monoclonal antibody (Product # M551) in duplicate at 1, 2, 4, 5, 7, and 9 µg/mL for at least 12 hours. The plate was blocked with a proprietary blocking solution for 1-4 hours at room temperature, and 50 µL of mouse IL-5 recombinant protein (Product # SMIL5) was added in replicates of twelve at 400, 160, 64, 25.6, 10.24, and 0pg/mL for 1 hour at room temperature. The plate was washed and incubated 50 µL of a biotinylated IL-5... View More
产品信息
M550B
产品规格
种属反应
Human
宿主/亚型
Rat
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
39D10
抗原
Recombinant human IL-5
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Caprylic acid precipitation
保存液
PBS with 4% BSA
内含物
proprietary Preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_223573
产品详细信息
M550B targets IL-5 in ELISA, and WB applications and shows reactivity with Human samples.
The M550B immunogen is recombinant human IL-5.
M550B detects IL-5 which has a predicted molecular weight of approximately 13 kDa.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The M550B IL1b antibody (biotinylated conjugate of clone 39D10) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M551 (clone 5A10). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Biotinylated antibody M550B (clone 39D10) and antibody M551 (clone 5A10) have successfully been used in combination with recombinant IL5 protein SIL5 in ELISA applications.
靶标信息
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: B-cell differentiation factor I; colony-stimulating factor, eosinophil; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF
基因别名: EDF; IL-5; IL5; TRF
UniProt ID: (Human) P05113
Entrez Gene ID: (Human) 3567
MAPK cascade
inflammatory response
immune response
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
positive regulation of B cell proliferation
positive regulation of GTPase activity
positive regulation of eosinophil differentiation
positive regulation of transcription, DNA-templated
positive regulation of JAK-STAT cascade
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of immunoglobulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of podosome assembly
