Target of Interest
Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
图: 1 / 3
IL-9 Antibody (500-P29-1MG) in WB
Western Blot: To detect Human IL-9 by Western Blot analysis IL-9 Polyclonal Antibody (Product # 500-P29-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P29-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human IL-9
偶联物
Unconjugated
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930264
产品详细信息
AA Sequence of recombinant protein: MQGCPTLAGI LDINFLINKM QEDPASKCHC SANVTSCLCL GIPSDNCTRP CFSERLSQMT NTTMQTRYPL IFSRVKKSVE VLKNNKCPYF SCEQPCNQTT AGNALTFLKS LLEIFQKEKM RGMRGKI.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-9. Anti-Human IL-9-specific antibody was purified by affinity chromatography employing an immobilized Human IL-9 matrix.
Sandwich ELISA: To detect Human IL-9 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-9 (500-P29Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-9.
Western Blot: To detect Human IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cytokine P40; homolog of mouse T cell and mast cell growth factor 40; IL-9; ILN; Interleukin; Interleukin-9; Interleukin9; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40
基因别名: HP40; IL-9; IL9; P40
UniProt ID: (Human) P15248
Entrez Gene ID: (Human) 3578
