Invitrogen

IL17RA Monoclonal Antibody (133617)

IL17RA Antibody in Flow Cytometry (Flow)

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IL17RA Antibody (MA5-23864) in Flow

Knockdown validation by Flow cytometry of IL17RA in IL-17 RA/IL-17 R knockout K562 human myelogenous leukemia cell line. Samples were incubated in IL17RA monoclonal antibody (Product # MA5-23864) followed by a anti-Mouse IgG PE-conjugated secondary antibody. Cell line was stained with monoclonal antibody or isotype control antibody. No staining in the IL-17 RA/IL-17 R knockout K562 cell line was observed.

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产品信息

MA5-23864

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

流式细胞分析 (Flow)

0.25 µg per million cells

中和实验 (Neu)

Assay-Dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

133617

抗原

Mouse myeloma cell line NS0-derived recombinant human IL-17RA/IL-17R Leu33-Trp320

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2606115

产品详细信息

In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL-17B R, or recombinant human IL-17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

靶标信息

Interleukin 17A (IL-17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17RA is a ubiquitous type I membrane glycoprotein that binds with low affinity to IL-17A and IL-17F. IL-17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Defects in IL-17RA are the cause of familial candidiasis type 5 (CANDF5) which is a rare disorder with altered immune responses and impaired clearance of fungal infections.

仅用于科研。不用于诊断过程。未经明确授权不得转售。