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Invitrogen

INSR Monoclonal Antibody (CT-3)

高级验证

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8 篇参考文献

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说明书

实验方案

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货号 MA5-13783

CNY 4,978.00

500 µL

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说明书

实验方案

技术支持

Application

INSR Antibody in Immunocytochemistry (ICC/IF)

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INSR Antibody (MA5-13783) in ICC/IF

Immunofluorescence analysis of Insulin receptor was performed using 70% confluent log phase Reh cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with INSR Monoclonal Antibody (CT-3) (Product # MA5-13783) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 ... View More

INSR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

INSR Antibody in Immunohistochemistry (IHC)

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INSR Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across Reh and Jurkat owing to their inherent genetic constitution. Relative expression of Insulin receptor was observed ~100 kDa in Reh along with the proinsulin receptor ~180 kDa versus no pick up in Jurkat using Anti-INSR Monoclonal Antibody (CT-3) (Product # MA5-13783) in Western Blot. {RE}

产品信息

MA5-13783

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:50

免疫细胞化学 (ICC/IF)

1:10-1:100

酶联免疫吸附实验 (ELISA)

Assay-dependent

亲和纯化 (AP)

Assay-dependent

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

CT-3

抗原

Recombinant-fragment including the C-terminal 100 amino acids of human insulin receptor

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偶联物

Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10985120

产品详细信息

MA5-13783 targets Insulin Receptor beta in ELISA, ICC/IF, IHC (P), Affinity Purification, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.

The MA5-13783 immunogen is recombinant-fragment including the C-terminal 100 amino acids of human insulin receptor.

靶标信息

INSR is a receptor tyrosine kinase. Binding of insulin to the extracellular domain of INSR stimulates glucose uptake. INSR and IGF-1 receptors share major structural and functional similarity. The earliest cellular response to insulin stimulation is autophosphorylation of tyrosine in INSR. In humans, the INSR gene is located on chromosome 19. Defects in INSR are the cause of various insulin resistance syndromes and IGF-1R defects may also cause some forms of growth retardation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Investigating the role of the IGF axis as a predictor of biochemical recurrence in prostate cancer patients post-surgery.

The Prostate

Breen KJ,O'Neill A,Murphy L,Fan Y,Boyce S,Fitzgerald N,Dorris E,Brady L,Finn SP,Hayes BD,Treacy A,Barrett C,Aziz MA,Kay EW,Fitzpatrick JM,Watson RWG

Published figure using INSR monoclonal antibody (Product # MA5-13783) in Immunohistochemistry

Fri Sep 01 00:00:00 UTC 2017

Hyperphosphorylation of Tau induced by naturally secreted amyloid-β at nanomolar concentrations is modulated by insulin-dependent Akt-GSK3β signaling pathway.

The Journal of biological chemistry

Tokutake T,Kasuga K,Yajima R,Sekine Y,Tezuka T,Nishizawa M,Ikeuchi T

MA5-13783 was used in western blot to study the role of insulin-dependent Akt/GSK3-beta signaling in the mechanism by which nanomolar levels of secreted beta-amyloid induce tau hyperphosphorylation in recipient cells

Fri Oct 12 00:00:00 UTC 2012

Selective response to insulin versus insulin-like growth factor-I and -II and up-regulation of insulin receptor splice variant B in the differentiated mouse mammary epithelium.

Endocrinology

Berlato C,Doppler W

MA5-13783 was used in western blot to investigate the role of insulin receptor splice variant B in the differentiated mammary epithelium

Mon Jun 01 00:00:00 UTC 2009

Inhibitory effects of nordihydroguaiaretic acid (NDGA) on the IGF-1 receptor and androgen dependent growth of LAPC-4 prostate cancer cells.

The Prostate

Ryan CJ,Zavodovskaya M,Youngren JF,Campbell M,Diamond M,Jones J,Shiry L,Allan G,Maddux BA,Goldfine ID

MA5-13783 was used in western blot to study the inhibition of IGF-1 receptor and androgen-dependent growth of prostate cancer cells by nordihydroguaiaretic acid

Fri Aug 01 00:00:00 UTC 2008

Inhibition by transmembrane peptides of chimeric insulin receptors.

Cellular and molecular life sciences : CMLS

Bennasroune A,Gardin A,Auzan C,Clauser E,Dirrig-Grosch S,Meira M,Appert-Collin A,Aunis D,Crémel G,Hubert P

MA5-13783 was used in western blot to study the ability of EGFR and ErbB2 receptor transmembrane peptides to inhibit the autophosphorylation of chimeric insulin receptors containing these sequences

Thu Sep 01 00:00:00 UTC 2005

The use of protein tyrosine phosphatase 1B and insulin receptor immunostains to differentiate nonalcoholic from alcoholic steatohepatitis in liver biopsy specimens.

American journal of clinical pathology

Sanderson SO,Smyrk TC

MA5-13783 was used in immunohistochemistry to evaluate protein tyrosine phosphatase 1B and insulin receptor as markers for differentiating nonalcoholic and alcoholic steatohepatitis

Fri Apr 01 00:00:00 UTC 2005

Coordinated regulation of insulin signaling by the protein tyrosine phosphatases PTP1B and TCPTP.

Molecular and cellular biology

Galic S,Hauser C,Kahn BB,Haj FG,Neel BG,Tonks NK,Tiganis T

MA5-13783 was used in western blot to study the coordinated regulation of insulin signaling by the protein tyrosine phosphatases PTP1B and TCPTP

Sat Jan 01 00:00:00 UTC 2005

Focal overexpression of insulin-like growth factor 2 by hepatocytes and cholangiocytes in viral liver cirrhosis.

British journal of cancer

Sedlaczek N,Hasilik A,Neuhaus P,Schuppan D,Herbst H

MA5-13783 was used in immunohistochemistry to study IGF-2 focal overexpression by hepatocytes and cholangiocytes in viral liver cirrhosis

Mon Mar 10 00:00:00 UTC 2003

生物信息学

蛋白别名: CD 220; CD220; CD220 antigen; CD220 beta; CD221; HHF 5; IGF-I receptor; Insulin receptor; IR; IR 1; IR beta; IR-1; IR1

基因别名: 4932439J01Rik; CD220; D630014A15Rik; HHF5; INSR; IR; IR-A; IR-B

UniProt ID: (Human) P06213, (Mouse) P15208

Entrez Gene ID: (Human) 3643, (Mouse) 16337

功能

protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity insulin-activated receptor activity insulin-like growth factor receptor binding protein binding ATP binding GTP binding protein phosphatase binding lipoic acid binding insulin-like growth factor I binding insulin-like growth factor II binding protein complex binding 3-phosphoinositide-dependent protein kinase binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding PTB domain binding nucleotide binding protein kinase activity transmembrane receptor protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding protein domain specific binding transmembrane signal receptor

参与通路

INSR Monoclonal Antibody (CT-3)

INSR Antibody (MA5-13783) in ICC/IF

产品信息

MA5-13783

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

亲和纯化 (AP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

INSR Monoclonal Antibody (CT-3)

INSR Antibody (MA5-13783) in ICC/IF

产品信息

MA5-13783

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

亲和纯化 (AP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID