Invitrogen

IRF4 Monoclonal Antibody (3E4), PE-eFluor™ 610, eBioscience™

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IRF4 Antibody (61-9858-82) in Flow

Normal human peripheral blood cells were stimulated for 3 days with Anti-Human CD3 and Anti-Human CD28 Functional Grade Purifieds (Product # 16-0037-81 and Product # 16-0289-81) and then intracellularly stained with 0.25 µg of Rat IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4301-82) (blue histogram) or 0.25 µg of Anti-Human/Mouse IRF4 PE-eFluor® 610 (purple histogram) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. The orange histogram is unstimulated cells stained with 0.25 µg of Anti-... View More

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产品信息

61-9858-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse, Rhesus monkey

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

3E4

偶联物

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

激发/发射光谱

565/606 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2637108

产品详细信息

Description: The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4). IRF4 is a 52 kDa transcription factor with roles in B cell, T cell and macrophage function. In B cells, IRF4 is highly expressed in mature plasma cells and plays a crucial role in their differentiation. IRF4 has been shown to interact with PU.1 and control the transcription of many B cell-specific genes including Prdm1, which encodes Blimp1. In T cells, IRF4 has been implicated in regulatory T (Treg), Th2, Th9 and Th17 cell development and function. This transcription factor is upregulated upon T cell activation and is expressed in mature T cells. Studies have shown that IRF4 directly induces Blimp1 expression in Tregs, leading to IL-10 expression. IRF4 has also been demonstrated to be involved in macrophage polarization and regulation. Lastly, in addition to its roles in normal immune function, IRF4 expression has been reported to be upregulated in many blood-related cancers.

Applications Reported: This 3E4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 3E4 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses.

Nature communications

Lam JH,Baumgarth N

Published figure using IRF4 monoclonal antibody (Product # 61-9858-82) in Flow Cytometry

Wed Jul 05 00:00:00 EDT 2023

B cells promote CD8 T cell primary and memory responses to subunit vaccines.

Cell reports

Klarquist J,Cross EW,Thompson SB,Willett B,Aldridge DL,Caffrey-Carr AK,Xu Z,Hunter CA,Getahun A,Kedl RM

Published figure using IRF4 monoclonal antibody (Product # 61-9858-82) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Limited access to antigen drives generation of early B cell memory while restraining the plasmablast response.

Immunity

Glaros V,Rauschmeier R,Artemov AV,Reinhardt A,Ols S,Emmanouilidi A,Gustafsson C,You Y,Mirabello C,Björklund ÅK,Perez L,King NP,Månsson R,Angeletti D,Loré K,Adameyko I,Busslinger M,Kreslavsky T

61-9858-82 was used in Flow cytometry/Cell sorting to examine the early B cell response to T-dependent antigen in mice by single-cell RNA sequencing.

Tue Sep 14 00:00:00 EDT 2021

Limited access to antigen drives generation of early B cell memory while restraining the plasmablast response.

Immunity

Glaros V,Rauschmeier R,Artemov AV,Reinhardt A,Ols S,Emmanouilidi A,Gustafsson C,You Y,Mirabello C,Björklund ÅK,Perez L,King NP,Månsson R,Angeletti D,Loré K,Adameyko I,Busslinger M,Kreslavsky T

61-9858-82 was used in Flow cytometry/Cell sorting to examine the early B cell response to T-dependent antigen in mice by single-cell RNA sequencing.

Tue Sep 14 00:00:00 EDT 2021

Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells.

Cell reports

Shin B,Benavides GA,Geng J,Koralov SB,Hu H,Darley-Usmar VM,Harrington LE

Published figure using IRF4 monoclonal antibody (Product # 61-9858-82) in Flow Cytometry

Tue Feb 11 00:00:00 EST 2020

Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR.

Nature communications

Zhu H,Bhatt B,Sivaprakasam S,Cai Y,Liu S,Kodeboyina SK,Patel N,Savage NM,Sharma A,Kaufman RJ,Li H,Singh N

Published figure using IRF4 monoclonal antibody (Product # 61-9858-82) in Flow Cytometry

Wed Mar 06 00:00:00 EST 2019

ROCK2, but not ROCK1 interacts with phosphorylated STAT3 and co-occupies TH17/TFH gene promoters in TH17-activated human T cells.

Scientific reports

Chen W,Nyuydzefe MS,Weiss JM,Zhang J,Waksal SD,Zanin-Zhorov A

Published figure using IRF4 monoclonal antibody (Product # 61-9858-82) in Flow Cytometry

Fri Nov 09 00:00:00 EST 2018

Batf3 selectively determines acquisition of CD8⁺ dendritic cell phenotype and function.

Immunology and cell biology

Chandra J,Kuo PT,Hahn AM,Belz GT,Frazer IH

Published figure using IRF4 monoclonal antibody (Product # 61-9858-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

γ-Tocopherol supplementation of allergic female mice augments development of CD11c+CD11b+ dendritic cells in utero and allergic inflammation in neonates.

American journal of physiology. Lung cellular and molecular physiology

Abdala-Valencia H,Soveg F,Cook-Mills JM

Published figure using IRF4 monoclonal antibody (Product # 61-9858-82) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using IRF4 monoclonal antibody (Product # 61-9858-82) in Flow Cytometry

Mon Jul 04 00:00:00 EDT 2016

Cell-intrinsic expression of TLR9 in autoreactive B cells constrains BCR/TLR7-dependent responses.

Journal of immunology (Baltimore, Md. : 1950)

Nündel K,Green NM,Shaffer AL,Moody KL,Busto P,Eilat D,Miyake K,Oropallo MA,Cancro MP,Marshak-Rothstein A

Published figure using IRF4 monoclonal antibody (Product # 61-9858-82) in Flow Cytometry

Sun Mar 15 00:00:00 EDT 2015

Beta-catenin signaling drives differentiation and proinflammatory function of IRF8-dependent dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Cohen SB,Smith NL,McDougal C,Pepper M,Shah S,Yap GS,Acha-Orbea H,Jiang A,Clausen BE,Rudd BD,Denkers EY

Published figure using IRF4 monoclonal antibody (Product # 61-9858-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

生物信息学

蛋白别名: Interferon regulatory factor 4; IRF-4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; NF-EM5; PU.1 interaction partner; Sfpi1/PU.1 interaction partner; Transcriptional activator PIP

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基因别名: AI385587; IRF-4; IRF4; LSIRF; MUM1; NF-EM5; SHEP8; Spip

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UniProt ID: (Human) Q15306, (Mouse) Q64287

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Entrez Gene ID: (Human) 3662, (Mouse) 16364

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein-lysine N-methyltransferase activity sequence-specific DNA binding regulatory region DNA binding DNA binding winged helix/forkhead transcription factor

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