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IRF4 Antibody (MA5-35677) in WB
Western blot analysis of IRF4 in extracts of various cell lines. Samples were incubated with IRF4 Monoclonal antibody (Product # MA5-35677) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
MA5-35677
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
ARC1261
抗原
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IRF4 (Q15306)
偶联物
形式
Liquid
浓度
0.47 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2849577
产品详细信息
Immunogen sequence: MNLEGGGRGG EFGMSAVSCG NGKLRQWLID QIDSGKYPGL VWENEEKSIF RIPWKHAGKQ DYNREEDAAL FKAWALFKGK FREGIDKPDP PTWKTRLRCA
靶标信息
The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Interferon regulatory factor 4; IRF-4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; NF-EM5
基因别名: IRF4; LSIRF; MUM1; NF-EM5; SHEP8
UniProt ID: (Human) Q15306
Entrez Gene ID: (Human) 3662
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
protein-lysine N-methyltransferase activity
sequence-specific DNA binding
winged helix/forkhead transcription factor
transcription from RNA polymerase II promoter
peptidyl-lysine methylation
negative regulation of toll-like receptor signaling pathway
T cell activation
defense response to protozoan
myeloid dendritic cell differentiation
positive regulation of DNA binding
histone H3 acetylation
histone H4 acetylation
positive regulation of interleukin-10 biosynthetic process
positive regulation of interleukin-2 biosynthetic process
positive regulation of interleukin-13 biosynthetic process
positive regulation of interleukin-4 biosynthetic process
regulation of T-helper cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
T-helper 17 cell lineage commitment
