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IRF5 Antibody (50-9698-42) in Flow
Normal human peripheral blood cells were stained with Anti-Human CD3 PerCP-eFluor® 710 (Product # 46-0036-42), Anti-Human CD11b FITC (Product # 11-0118-42), Anti-Human CD123 PE (Product # 12-1239-42), and Fixable Viability Dye eFluor® 780 (Product # 65-0865-14). Intracellular staining with Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or Anti-Human IRF5 eFluor® 660 (right) was performed using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Viable single cells in the CD3+ (ora... View More
产品信息
50-9698-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
ALYSCLN
偶联物
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2574347
产品详细信息
Description: The ALYSCLN monoclonal antibody reacts with human IRF5, a 60-63 kDa member of the interferon regulatory factor family of transcription factors that have diverse roles in host defense against viruses. IRF5 was originally identified as an important regulator of IFN alpha and IFN beta and more recently has been shown to be regulated by these interferons. IRF5 is also involved in induction of pro-inflammatory cytokines including IL-6, IL-12 and TNF alpha in response to TLR signaling. Specifically, it has been shown that MyD88 and TRAF6 interact directly with IRF5 in response to TLR activation and this interaction results in IRF5 nuclear translocation. Additionally, IRF5 has been shown to have both p53-dependent and independent effects in regulation of cell cycle and apoptosis. Genetic polymorphisms in the IRF5 locus have been implicated in many autoimmune diseases including Sjogren's syndrome, inflammatory bowel disease, multiple sclerosis and systemic lupus erythematosus.
Recently, a role for IRF5 in the polarization of classically activated (M1) macrophages has been identified. In these macrophages, IRF5 directly upregulates expression of IL-12 and IL-23 and represses IL-10. Moreover, exogenous expression of IRF5 in M2-polarized (alternatively activated) macrophages induces expression of M1-related cytokines and chemokines. IRF5 is expressed at highest levels in monocytes, macrophages and plasmacytoid dendritic cells, but is also expressed in B cells and T cells.
Applications Reported: This ALYSCLN antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This ALYSCLN antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: Interferon regulatory factor 5; IRF-5; OTTHUMP00000212907
基因别名: IRF5; SLEB10
UniProt ID: (Human) Q13568
Entrez Gene ID: (Human) 3663
transcription, DNA-templated
response to peptidoglycan
response to muramyl dipeptide
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-12 production
positive regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
