Invitrogen

ISG15 Recombinant Rabbit Monoclonal Antibody (1H9L21)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4个已发表图片

3 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

ISG15 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

ISG15 Antibody (703132) in ICC/IF

For immunofluorescence analysis, RAW 264.7 cells were fixed and permeabilized for detection of endogenous ISG15 (Mouse) using Anti-ISG15 (Mouse) Recombinant Rabbit Monoclonal Antibody (Product # 703132, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a... View More

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ISG15 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. NIH/3T3 cells were treated with IFN-beta (1000 U/mL for 24 hrs) and were transfected with ISG15 Mouse siRNA and a decrease in signal intensity was observed in Western blot application using Anti-ISG15 (Mouse) Recombinant Rabbit Monoclonal Antibody (Product # 703132). {KD}

产品信息

703132

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

1H9L21

抗原

Protein corresponding to mouse ISG15 [aa2-aa155]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2784563

产品详细信息

This antibody is predicted to react with Rat, Cat.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Pressure overload induces ISG15 to facilitate adverse ventricular remodeling and promote heart failure.

The Journal of clinical investigation

Yerra VG,Batchu SN,Kaur H,Kabir MDG,Liu Y,Advani SL,Tran DT,Sadeghian S,Sedrak P,Billia F,Kuzmanov U,Gramolini AO,Qasrawi DO,Petrotchenko EV,Borchers CH,Connelly KA,Advani A

Published figure using ISG15 recombinant monoclonal antibody (Product # 703132) in Western Blot

Mon May 01 00:00:00 EDT 2023

CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states.

Nature neuroscience

Leng K,Rose IVL,Kim H,Xia W,Romero-Fernandez W,Rooney B,Koontz M,Li E,Ao Y,Wang S,Krawczyk M,Tcw J,Goate A,Zhang Y,Ullian EM,Sofroniew MV,Fancy SPJ,Schrag MS,Lippmann ES,Kampmann M

703132 was used in Immunohistochemistry (Frozen) to find that autocrine-paracrine IL-6 and interferon signaling downstream of canonical NF-κB activation drove two distinct inflammatory reactive signatures, one promoted by STAT3 and the other inhibited by STAT3.

Tue Nov 01 00:00:00 EDT 2022

In Vivo Profiling of Individual Multiciliated Cells during Acute Influenza A Virus Infection.

Journal of virology

Hamele CE,Russell AB,Heaton NS

703132 was used in Immunohistochemistry (Frozen) to propose that different ratios of these individual cellular responses may contribute to the broader antiviral state of the lung and may ultimately affect disease severity.

Wed Jul 27 00:00:00 EDT 2022

生物信息学

蛋白别名: interferon, alpha-inducible protein; Interferon-induced 15 kDa protein; interferon-induced 15-KDa protein; Interferon-induced 17 kDa protein; interferon-stimulated protein (15 kDa); interferon-stimulated protein 15; IP17; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15

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基因别名: G1p2; IGI15; IP17; Irfp; Isg15; UCRP

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UniProt ID: (Mouse) Q64339

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Entrez Gene ID: (Mouse) 100038882

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功能

protein binding protein tag

参与通路

modification-dependent protein catabolic process negative regulation of protein ubiquitination ISG15-protein conjugation regulation of interferon-gamma production response to type I interferon defense response to bacterium negative regulation of viral genome replication positive regulation of erythrocyte differentiation defense response to virus