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Application
FIGURE: 1 / 2
Ihh Antibody (PA5-115399) in ICC/IF
Immunocytochemistry analysis of Ihh in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115399). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115399
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human IHH(Accession Q14623), corresponding to amino acid residues K108-R158.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2900035
Product Specific Information
Antibody detects endogenous levels of total IHH.
Target Information
Ihh encodes a member of the hedgehog family of secreted signaling molecules. Hedgehog proteins are essential regulators of a variety of developmental processes including growth, patterning and morphogenesis. The encoded protein specifically plays a role in bone growth an differentiation. Mutations in this gene are the cause of brachydactyly type A1 which is characterized by shortening or malformation of the phalanges. Mutations in this gene are also the cause of acrocapitofemoral dysplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HHG-2; IHH; Indian hedgehog homolog; Indian hedgehog protein
Gene Aliases: BDA1; HHG2; IHH
UniProt ID: (Human) Q14623, (Mouse) P97812
Entrez Gene ID: (Human) 3549, (Mouse) 16147, (Rat) 84399
skeletal system development
ossification
patterning of blood vessels
osteoblast differentiation
in utero embryonic development
cell fate specification
morphogenesis of a branching structure
vasculature development
heart looping
positive regulation of mesenchymal cell proliferation
epithelial cell morphogenesis
retinal pigment epithelium development
proteoglycan metabolic process
proteolysis
cell communication
smoothened signaling pathway
cell-cell signaling
multicellular organism development
pattern specification process
positive regulation of cell proliferation
embryo development
embryonic pattern specification
negative regulation of signal transduction
intein-mediated protein splicing
cell differentiation
vitelline membrane formation
pancreas development
positive regulation of chondrocyte differentiation
positive regulation of collagen biosynthetic process
negative regulation of T cell differentiation in thymus
negative regulation of immature T cell proliferation in thymus
positive regulation of T cell differentiation in thymus
multicellular organism growth
chondrocyte proliferation
regulation of growth
embryonic digit morphogenesis
camera-type eye development
negative regulation of apoptotic process
bone resorption
regulation of cell differentiation
negative regulation of cell differentiation
positive regulation of smoothened signaling pathway
positive regulation of transcription from RNA polymerase II promoter
positive regulation of alpha-beta T cell differentiation
negative regulation of alpha-beta T cell differentiation
negative regulation of eye pigmentation
cell maturation
embryonic digestive tract morphogenesis
embryonic camera-type eye morphogenesis
neuron development
smooth muscle tissue development
positive regulation of epithelial cell proliferation
cartilage development
camera-type eye photoreceptor cell fate commitment
head morphogenesis
somite development
embryonic skeletal joint development
epithelial cell-cell adhesion
response to estradiol
maternal process involved in female pregnancy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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