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Importin 4 Antibody (PA5-100069) in ICC/IF
Immunofluorescent analysis of Importin 4 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Importin 4 polyclonal antibody (Product # PA5-100069) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 4... View More
产品信息
PA5-100069
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human IPO4(Accession Q8TEX9), corresponding to amino acid residues L440-N490.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2815599
产品详细信息
Antibody detects endogenous levels of total IPO4.
靶标信息
Importin-4 is a protein that in humans is encoded by the IPO4 gene. Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Imp4; Imp4a; Imp4b; importin 4a; Importin-4; Importin-4a; Importin-4b; Ran-binding protein 4; RanBP4
基因别名: 8430408O15Rik; AA409693; Imp4; Imp4a; IMP4B; IPO4; RANBP4
UniProt ID: (Human) Q8TEX9, (Mouse) Q8VI75
Entrez Gene ID: (Human) 79711, (Rat) 290228, (Mouse) 75751
protein import into nucleus, docking
protein import into nucleus, translocation
DNA replication-dependent nucleosome assembly
DNA replication-independent nucleosome assembly
NLS-bearing protein import into nucleus
ribosomal protein import into nucleus
protein transport
intracellular protein transport
transport
ribosome biogenesis
