Invitrogen

Integrin alpha 4 beta 7 (LPAM-1) Monoclonal Antibody (DATK32 (DATK-32)), Functional Grade, eBioscience™

Integrin alpha 4 beta 7 (LPAM-1) Antibody in Flow Cytometry (Flow)

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Integrin alpha 4 beta 7 (LPAM-1) Antibody (16-5887-85) in Flow

Staining of C57Bl/6 splenocytes with 0.5 µg of Rat IgG2a Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.5 µg of Anti-Mouse Integrin alpha 4 beta 7 (LPAM-1) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

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产品信息

16-5887-85

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

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中和实验 (Neu)

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功能检测 (Functional)

Assay-Dependent

抑制试剂 (IA)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

DATK32 (DATK-32)

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_657808

产品详细信息

Description: The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha4beta7), which is a heterodimer of the the 154 kDa alpha4 subunit and the 130 kDa beta7 subunit. In mouse, the beta7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion.

Applications Reported: This DATK32 (DATK-32) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DATK32 (DATK-32) antibody has been tested tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Critical role of plasmacytoid dendritic cells in induction of oral tolerance.

The Journal of allergy and clinical immunology

Uto T,Takagi H,Fukaya T,Nasu J,Fukui T,Miyanaga N,Arimura K,Nakamura T,Choijookhuu N,Hishikawa Y,Sato K

16-5887 was used in Flow cytometry/Cell sorting to determine the role of plasmacytoid dendritic cells (pDCs).

Fri Jun 01 00:00:00 EDT 2018

Flt3 ligand expands bona fide innate lymphoid cell precursors in vivo.

Scientific reports

Parigi SM,Czarnewski P,Das S,Steeg C,Brockmann L,Fernandez-Gaitero S,Yman V,Forkel M,Höög C,Mjösberg J,Westerberg L,Färnert A,Huber S,Jacobs T,Villablanca EJ

16-5887 was used in Flow cytometry/Cell sorting to indicate that Flt3L expands CHILPs in the bone marrow, which might be associated with specific inflammatory conditions.

Tue Jan 09 00:00:00 EST 2018

WASH maintains NKp46⁺ ILC3 cells by promoting AHR expression.

Nature communications

Xia P,Liu J,Wang S,Ye B,Du Y,Xiong Z,Han ZG,Tong L,Fan Z

16-5887 was used in Flow cytometry/Cell sorting to study how WASH-mediated AHR expression has a critical function in the maintenance of NKp46(+) ILC3s.

Wed Jun 07 00:00:00 EDT 2017

β7 Integrin controls mast cell recruitment, whereas αE integrin modulates the number and function of CD8+ T cells in immune complex-mediated tissue injury.

Journal of immunology (Baltimore, Md. : 1950)

Yamada D,Kadono T,Masui Y,Yanaba K,Sato S

16-5887 was used in Inhibition experiments to assess the role of αE and Β7 integrin in immune complex-mediated tissue injury.

Thu May 01 00:00:00 EDT 2014

Peripheral tissue homing receptors enable T cell entry into lymph nodes and affect the anatomical distribution of memory cells.

Journal of immunology (Baltimore, Md. : 1950)

Brinkman CC,Rouhani SJ,Srinivasan N,Engelhard VH

16-5887 was used in Flow cytometry/Cell sorting to characterise the expression of different cell surface markers on T cells present within different regions of lymph nodes.

Sun Sep 01 00:00:00 EDT 2013

Lymphocytes in the peritoneum home to the omentum and are activated by resident dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Carlow DA,Gold MR,Ziltener HJ

16-5887 was used in Blocking experiments to show that B and T lymphocytes counterflow from the peritoneal cavity to the omentum in mice where they are activated by resident dendritic cells.

Wed Jul 15 00:00:00 EDT 2009

生物信息学

蛋白别名: CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; Integrin beta-7; Integrin beta-P; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; M290 IEL antigen; VLA; VLA 4; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA4 subunit alpha

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基因别名: CD49D; Itga4; Itga4B; Itgb7; Ly69

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UniProt ID: (Mouse) Q00651, (Mouse) P26011

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Entrez Gene ID: (Mouse) 16401, (Mouse) 16421

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功能

fibronectin binding metal ion binding cell adhesion molecule binding protein antigen binding receptor activity integrin

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