Invitrogen

Integrin alpha 4 beta 7 (LPAM-1) Monoclonal Antibody (DATK32 (DATK-32)), APC, eBioscience™

Integrin alpha 4 beta 7 (LPAM-1) Antibody in Flow Cytometry (Flow)

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Integrin alpha 4 beta 7 (LPAM-1) Antibody (17-5887-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Mouse Integrin alpha 4 beta 7 (LPAM-1) APC (right). Total viable cells were used for analysis.

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产品信息

17-5887-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

DATK32 (DATK-32)

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1210577

产品详细信息

Description: The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha 4/ beta 7), which is a heterodimer of the the 154 kDa alpha 4 subunit and the 130 kDa beta 7 subunit. In mouse, the beta 7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion.

Applications Reported: This DATK32 (DATK-32) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DATK32 (DATK-32) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Epithelial colonization by gut dendritic cells promotes their functional diversification.

Immunity

Rivera CA,Randrian V,Richer W,Gerber-Ferder Y,Delgado MG,Chikina AS,Frede A,Sorini C,Maurin M,Kammoun-Chaari H,Parigi SM,Goudot C,Cabeza-Cabrerizo M,Baulande S,Lameiras S,Guermonprez P,Reis e Sousa C,Lecuit M,Moreau HD,Helft J,Vignjevic DM,Villablanca EJ,Lennon-Duménil AM

17-5887-82 was used in Flow cytometry/Cell sorting to investigate How tissue-specific cues orchestrate Dendritic cells diversification.

Tue Jan 11 00:00:00 EST 2022

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

17-5887-82 was used in Flow Cytometry to suggest a revised model for ILC differentiation that redefines the cell-fate potential of helper-ILC-restricted Zbtb16+ ILCPs.

Tue Apr 16 00:00:00 EDT 2019

DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation.

Nature medicine

Xiao Q,Wu J,Wang WJ,Chen S,Zheng Y,Yu X,Meeth K,Sahraei M,Bothwell ALM,Chen L,Bosenberg M,Chen J,Sexl V,Sun L,Li L,Tang W,Wu D

17-5887 was used in Flow cytometry/Cell sorting to identify a novel tumour immune-suppressive mechanism and immunotherapeutic targets particularly relevant for colorectal cancers and a subset of melanomas.

Thu Mar 01 00:00:00 EST 2018

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

17-5887 was used in Flow Cytometry to indicate that the VHL-HIF-glycolysis axis is essential for the late-stage maturation and function of ILC2s via targeting IL-33-ST2 pathway.

Tue Feb 20 00:00:00 EST 2018

Bcl11b is essential for licensing Th2 differentiation during helminth infection and allergic asthma.

Nature communications

Lorentsen KJ,Cho JJ,Luo X,Zuniga AN,Urban JF,Zhou L,Gharaibeh R,Jobin C,Kladde MP,Avram D

17-5887 was used in Flow cytometry/Cell sorting to establish novel roles for Bcl11b in the regulatory loop that licenses Th2 program in vivo.

Thu Apr 26 00:00:00 EDT 2018

Local HPV Recombinant Vaccinia Boost Following Priming with an HPV DNA Vaccine Enhances Local HPV-Specific CD8+ T-cell-Mediated Tumor Control in the Genital Tract.

Clinical cancer research : an official journal of the American Association for Cancer Research

Sun YY,Peng S,Han L,Qiu J,Song L,Tsai Y,Yang B,Roden RB,Trimble CL,Hung CF,Wu TC

17-5887 was used in Flow cytometry/Cell sorting to examine whether altering the administration method of TA-HPV vaccination can more effectively recruit antigen-specific T cells.

Mon Feb 01 00:00:00 EST 2016

Intravaginal HPV DNA vaccination with electroporation induces local CD8+ T-cell immune responses and antitumor effects against cervicovaginal tumors.

Gene therapy

Sun Y,Peng S,Qiu J,Miao J,Yang B,Jeang J,Hung CF,Wu TC

17-5887 was used in Flow cytometry/Cell sorting to examine the effects of a therapeutic HPV DNA vaccine administered through different routes with or without electroporation in an orthotopic HPV16 E7-expressing tumour mouse model.

Wed Jul 01 00:00:00 EDT 2015

Therapeutic evaluation of ex vivo-generated versus natural regulatory T-cells in a mouse model of chronic gut inflammation.

Inflammatory bowel diseases

Karlsson F,Martinez NE,Gray L,Zhang S,Tsunoda I,Grisham MB

17-5887 was used in Flow cytometry/Cell sorting to study the effect of ex vivo-generated and isolated Treg cells on a mouse model of chronic colitis, showing that ex-vivo Tregs were more potent at attenuating colitis, likely due to the associated decrease in IL-6 and IL-17A expression in the colon.

Tue Oct 01 00:00:00 EDT 2013

Homeostatic proliferation of naive CD4+ T cells in mesenteric lymph nodes generates gut-tropic Th17 cells.

Journal of immunology (Baltimore, Md. : 1950)

Kawabe T,Sun SL,Fujita T,Yamaki S,Asao A,Takahashi T,So T,Ishii N

17-5887 was used in Flow cytometry/Cell sorting to evaluate organ-specific naive CD4(+) T cell proliferation and differentiation in the lymphoid and intestinal tissues of mice.

Sat Jun 01 00:00:00 EDT 2013

Ex vivo generation of regulatory T cells: characterization and therapeutic evaluation in a model of chronic colitis.

Methods in molecular biology (Clifton, N.J.)

Karlsson F,Robinson-Jackson SA,Gray L,Zhang S,Grisham MB

17-5887 was used in Flow cytometry/Cell sorting to develop a relatively simple ex vivo method to generate large numbers of Foxp3-expressing iTregs.

Wed Feb 02 00:00:00 EST 2011

生物信息学

蛋白别名: CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; Integrin beta-7; Integrin beta-P; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; M290 IEL antigen; VLA; VLA 4; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA4 subunit alpha

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基因别名: CD49D; Itga4; Itga4B; Itgb7; Ly69

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UniProt ID: (Mouse) Q00651, (Mouse) P26011

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Entrez Gene ID: (Mouse) 16401, (Mouse) 16421

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功能

fibronectin binding metal ion binding cell adhesion molecule binding protein antigen binding receptor activity integrin

参与通路