Invitrogen

Integrin beta 7 Monoclonal Antibody (FIB504), PE-Cyanine7, eBioscience™

Integrin beta 7 Antibody in Flow Cytometry (Flow)

图: 1 / 2

Integrin beta 7 Antibody (25-5867-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0037-42) and Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or Anti-Mouse/Human Integrin Beta 7 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

25-5867-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

FIB504

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573481

产品详细信息

Description: The FIB504 monoclonal antibody reacts with human and mouse integrin beta 7. Integrin beta 7 is a 130 kD glycoprotein which associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Main ligands for integrin alpha 4 beta 7 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the main ligand of integrin alpha E beta 7 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response. The FIB504 antibody has been reported to have blocking activity.

Applications Reported: This FIB504 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This FIB504 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Multimodal cartography of human lymphopoiesis reveals B and T/NK/ILC lineages are subjected to differential regulation.

iScience

Alhaj Hussen K,Chabaane E,Nelson E,Lekiashvili S,Diop S,Keita S,Evrard B,Lardenois A,Delord M,Verhoeyen E,Cornils K,Kasraian Z,Macintyre EA,Cumano A,Garrick D,Goodhardt M,Andrieu GP,Asnafi V,Chalmel F,Canque B

25-5867-42 was used in Flow Cytometry to demonstrate that NK/ILC/T and B lineages are under fundamentally distinct modes of regulation.

Fri Oct 20 00:00:00 EDT 2023

Multimodal cartography of human lymphopoiesis reveals B and T/NK/ILC lineages are subjected to differential regulation.

iScience

25-5867-42 was used in Flow Cytometry to demonstrate that NK/ILC/T and B lineages are under fundamentally distinct modes of regulation.

Fri Oct 20 00:00:00 EDT 2023

Oral cholera vaccination promotes homing of IgA⁺ memory B cells to the large intestine and the respiratory tract.

Mucosal immunology

van Splunter M,van Hoffen E,Floris-Vollenbroek EG,Timmerman H,de Bos EL,Meijer B,Ulfman LH,Witteman B,Wells JM,Brugman S,Savelkoul HFJ,van Neerven RJJ

25-5867 was used in Flow cytometry/Cell sorting to reveal how the oral cholera vaccination can affect immune responses in other mucosal tissues, including the respiratory tract.

Sun Jul 01 00:00:00 EDT 2018

Intestinal helminth infection induces highly functional resident memory CD4⁺ T cells in mice.

European journal of immunology

Steinfelder S,Rausch S,Michael D,Kühl AA,Hartmann S

25-5867 was used in Flow cytometry/Cell sorting to show that nonlymphoid compartments can serve as reservoirs for Th2 memory cells, and furthermore that innate effector function of Th2 memory cells is restricted to CD4⁺ memory T cells residing in the peritoneal cavity.