Invitrogen

Integrin beta 7 Chimeric Recombinant Rabbit Monoclonal Antibody (FIB27)

Integrin beta 7 Chimeric Antibody in Immunocytochemistry (ICC/IF)

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Integrin beta 7 Chimeric Antibody (MA5-48015) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of Integrin beta 7 in human peripheral blood monocytes (PBMs) using Integrin beta 7 Chimeric Monoclonal Antibody (Product # MA5-48015). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with Integrin beta 7 Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor488 secondary antibody (1 µg/mL), showing membrane staining. ... View More

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产品信息

MA5-48015

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

10 µg/mL

流式细胞分析 (Flow)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

抑制试剂 (IA)

Assay-dependent

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

FIB27

抗原

FIB27 was prepared by immunizing rats with TK1 cells.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.02% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_3074458

产品详细信息

Specificity: FIB27 binds specifically to both human and mouse integrin beta-7 at epitope region Dl on which is involved in all a4b7-mediated adhesion events. Does not compete with DAKT32 for binding. FIB27 blocks LS722-induced TK1 cell aggregation (mAb LS722 is an activating anti-a4B7 antibody and induces aggregation via an a4B7-dependent pathway).

靶标信息

Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.

仅用于科研。不用于诊断过程。未经明确授权不得转售。