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JMY Antibody (A302-573A) in WB
Detection of human JMY by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-JMY antibody (Product # A302-573A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. JMY was more efficiently immunoprecipitated by rabbit anti-JMY antibody (Product # A302-572A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A an... View More
产品信息
A302-573A
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 800 and 850 of human junction mediating and regulatory protein.
偶联物
形式
Liquid
浓度
0.20 mg/mL
纯化类型
Antigen affinity chromatography
保存液
TBS, pH 7.0 to 8.0, with 0.1% BSA
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Wet ice
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
靶标信息
JMY acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ37870; Junction-mediating and -regulatory protein; junction-mediating and regulatory protein; WAS protein homology region 2 domain containing 1-like 3
基因别名: JMY; WHAMM2; WHDC1L3
UniProt ID: (Human) Q8N9B5
Entrez Gene ID: (Human) 133746
DNA repair
regulation of transcription from RNA polymerase II promoter
cell cycle arrest
Arp2/3 complex-mediated actin nucleation
positive regulation of apoptotic process
positive regulation of sequence-specific DNA binding transcription factor activity
'de novo' actin filament nucleation
actin polymerization-dependent cell motility
regulation of signal transduction by p53 class mediator
