Invitrogen

JNK2 Recombinant Rabbit Monoclonal Antibody (011)

JNK2 Antibody in Immunocytochemistry (ICC/IF)

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JNK2 Antibody (MA5-29409) in ICC/IF

Immunofluorescence staining of Human JNK2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with JNK2 Recombinant Rabbit Monoclonal Antibody (11) (Product # MA5-29409, 1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.

JNK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

JNK2 Antibody in Immunoprecipitation (IP)

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产品信息

MA5-29409

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:500-1:2,500

免疫细胞化学 (ICC/IF)

1:100-1:500

酶联免疫吸附实验 (ELISA)

1:25,000-1:50,000

免疫沉淀 (IP)

1-2 µL/mg of lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

011

抗原

Recombinant Human JNK2/MAPK9 protein (Met1-Arg424)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2785275

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Human JNK2/MAPK9.

靶标信息

MAPK9 (JNK2) is a serine/threonine kinase which plays an important role in the MAPK pathway. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: c-Jun kinase 2; c-Jun N-terminal kinase 2; JNK; JNK-55; JNK1; JNK1A2; JNK21B1/2; Jun kinase; MAP kinase 9; MAPK 10; MAPK 9; MAPK10; MAPK8; Mitogen-activated protein kinase 9; OTTHUMP00000161542; OTTHUMP00000161543; PRKM8; SAPK1; SAPK1a; Stress-activated protein kinase 1a; Stress-activated protein kinase JNK2

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基因别名: JNK-55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; MAPK9; p54a; p54aSAPK; PRKM9; SAPK; SAPK1A

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UniProt ID: (Human) P45984

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Entrez Gene ID: (Human) 5601

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功能

JUN kinase activity protein binding ATP binding transcription factor binding cysteine-type endopeptidase activator activity involved in apoptotic process mitogen-activated protein kinase kinase kinase binding non-receptor serine/threonine protein kinase

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