Invitrogen

K-Ras Monoclonal Antibody (9.13)

Name: K-Ras Monoclonal Antibody (9.13)
Brand: Invitrogen
SKU: 415700

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6 Published Figures

7 References

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Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

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Protocols

Questions & Answers

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K-Ras Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

K-Ras Antibody (415700) in ICC/IF

Immunofluorescence analysis of K-Ras was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with K-Ras (9.13) Mouse Monoclonal Antibody (Product # 415700) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a ... View More

K-Ras Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

K-Ras Antibody — Figure 2 miR-200c inhibits K-ras protein expression without affecting KRAS mRNA levels A) Luciferase reporter assay with different breast cancer cell lines. The renilla luciferase reporter containing the 3'UTR of KRAS including the predicted target site of miR-200c (RLuc) or the firefy luciferase control plasmid pGL3 (FLuc) were transfected into the indicated cell lines. Renilla reporter luciferase activity was normalized to the activity of the firefy control as ratio (RLuc/FLuc). Values are stated as mean +- SEM (n=5). B) Luciferase reporter assay upon miR-200c modulation. MDA-MB-436 cells were transfected with either pre-miR-200c (pre) or scrambled pre-miR-control (ctr). BT-474 cells were transfected with either miR-200c inhibitor (inh) or scrambled control inhibitor (ctr). Relative luciferase activities (RLuc/FLuc) are depicted in the graph. Values are stated as mean +- SEM (n=5). For statistical analysis a student's t-test was performed (**p<0.01; ***p<0.001). C) miR-200c inhibition in the miR-200c high cell lines BT-474 and MCF-7. Indicated cell lines were transfected with either miR-200c inhibitor (inh) or scrambled control inhibitor (ctr) and at 72h post transfection subjected to Western blot analysis (upper panel) or quantitative RT-PCR (lower panel). Values are stated as mean +- SD (n=3). D) miR-200c overexpression in the miR-200c low cell lines MDA-MB-231 and MDA-MB-436. Indicated cell lines were transfected with either pre-miR-200c (pre) or scrambled pre-miR-contro {KD}

Immunogen image

Immunogen Image K-Ras Monoclonal Antibody (9.13)

Product Details

415700

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1-2 µg/mL

Immunoprecipitation (IP)

5 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

9.13

Immunogen

Immunogen corresponds to aa 170-180 from P01116-1 (isoform 2A) of human K-Ras.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2532192

Product Specific Information

This antibody is specific for human K-Ras (K-Ras2, Ki-Ras, c-K-ras, GTPase KRas) protein (accession # NP_004976.2, P01116), which is 100% homologous with mouse, 95% with rat, and 94% with bovine, respectively. Reactivity has been confirmed on western blots with human HeLa and WI-38 cell lysates as well as rat KNRK and mouse NIH 3T3 cell lysates, and identifies the target band at~21 kDa. The reactivity has been also confirmed with rat KNRK cells using immunoprecipitation and with HeLa cells by immunofluorescence. Based on amino acid sequence homology, reactivity with bovine is also expected.

Target Information

K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: c-K-ras; c-K-ras2 protein; c-Ki-ras; c-Kirsten-ras protein; c-Kirsten-ras proto-oncogene; cellular c-Ki-ras2; cellular c-Ki-ras2 proto-oncogene; GTPase KRas; K RAS; K-Ras 2; K-ras p21 protein; Ki-Ras; Kirsten rat sarcoma oncogene 2, expressed; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral oncogene homologue 2 (active); oncogene KRAS2; p21 protein; PR310 c-K-ras oncogene; RASK; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

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Gene Aliases: AI929937; C-K-RAS; c-Ki-ras; CFC2; K-ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS; Kras-2; KRAS1; KRAS2; NS; NS3; p21; p21B; RALD; ras; RASK2

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UniProt ID: (Human) P01116, (Rat) P08644, (Mouse) P32883

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Entrez Gene ID: (Human) 3845, (Rat) 24525, (Mouse) 16653

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Function(s)

GTPase activity protein binding GTP binding GMP binding GDP binding LRR domain binding protein complex binding nucleotide binding small GTPase

Process(es)

MAPK cascade positive regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway epidermal growth factor receptor signaling pathway Ras protein signal transduction axon guidance positive regulation of cell proliferation visual learning positive regulation of gene expression cytokine-mediated signaling pathway forebrain astrocyte development actin cytoskeleton organization regulation of protein stability regulation of synaptic transmission, GABAergic positive regulation of Rac protein signal transduction social behavior endocrine signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway positive regulation of MAP kinase activity negative regulation of neuron apoptotic process negative regulation of cell differentiation regulation of long-term neuronal synaptic plasticity homeostasis of number of cells within a tissue leukocyte migration positive regulation of nitric-oxide synthase activity positive regulation of NF-kappaB transcription factor activity striated muscle cell differentiation response to glucocorticoid response to mineralocorticoid epithelial tube branching involved in lung morphogenesis small GTPase mediated signal transduction signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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K-Ras Monoclonal Antibody (9.13)

K-Ras Antibody (415700) in ICC/IF

Product Details

415700

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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