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Application
FIGURE: 1 / 3
KATNA1 Antibody (PA5-102975) in ICC/IF
Immunocytochemistry analysis of KATNA1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with KATNA1 polyclonal antibody (Product # PA5-102975) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102975
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KATNA1(Accession O75449), corresponding to amino acid residues E452-C491.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852349
Product Specific Information
Antibody detects endogenous levels of total KATNA1.
Target Information
Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. This gene encodes the p80 subunit. This protein is a member of the AAA family of ATPases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: katanin p60 (ATPase containing) subunit A 1; katanin p60 (ATPase-containing) subunit A 1; Katanin p60 ATPase-containing subunit A1; katanin p60 ATPase-containing subunit A1 {ECO:0000255|HAMAP-Rule:MF_03023}; katanin p60 subunit A 1; Katanin p60 subunit A1; katanin p60 subunit A1 {ECO:0000255|HAMAP-Rule:MF_03023}; KATNA1; lipotransin; microtubule severing protein; p60 katanin; p60 katanin {ECO:0000255|HAMAP-Rule:MF_03023}
Gene Aliases: KATNA1
UniProt ID: (Human) O75449, (Rat) Q6E0V2
Entrez Gene ID: (Human) 11104, (Rat) 292464, (Mouse) 23924
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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