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KCC2 Antibody (MA5-27610) in ICC/IF
Immunofluorescent analysis of KCC2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with KCC2 monoclonal antibody (Product # MA5-27610) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) KCC2 Antibody, D) Merged image. Magnification: 6... View More
产品信息
MA5-27610
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
N1/12
抗原
Fusion protein amino acids 932-1043 corresponding to rat KCC2
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2735237
产品详细信息
1 µg/mL of MA5-27610 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 140kDa.
This antibody was formerly sold as clone S1-12
靶标信息
Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Electroneutral potassium-chloride cotransporter 2; erythroid K-Cl cotransporter 2; Furosemide-sensitive K-Cl cotransporter; hKCC2; K-Cl cotransporter 2; KCC 2; KCC2a variant 1; KCC2a-S25 variant 1; KCC2b variant 2; mKCC2; Neuronal K-Cl cotransporter; neuronal-specific K-Cl cotransporter; rKCC2; S12A5; solute carrier family 12 (potassium/chloride transporter), member 5; Solute carrier family 12 member 5; solute carrier family 12, member 5
基因别名: EIEE34; EIG14; hKCC2; KCC2; KIAA1176; mKIAA1176; SLC12A5
UniProt ID: (Human) Q9H2X9, (Mouse) A2A5L0, (Rat) Q63633
Entrez Gene ID: (Human) 57468, (Mouse) 57138, (Rat) 171373
transport
ion transport
cellular ion homeostasis
chemical synaptic transmission
learning
cellular chloride ion homeostasis
multicellular organism growth
thermosensory behavior
response to drug
dendritic spine development
potassium ion transmembrane transport
chloride transmembrane transport
potassium ion transport
chloride transport
organic cation transport
ammonium transport
intracellular pH reduction
transmembrane transport
ammonium transmembrane transport
cation transmembrane transport
