Invitrogen

KHK Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他16个KHK抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (9)
高级验证 (2)

KHK Antibody in Immunocytochemistry (ICC/IF)

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KHK Antibody (PA5-29004) in ICC/IF

Immunofluorescence analysis of KHK was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KHK Polyclonal Antibody (Product # PA5-29004) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), 1:2000 d... View More

KHK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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KHK Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of Ketohexokinase was observed to be high in Mouse Liver, Rat Liver, Mouse kidney and Rat Kidney in comparison to low or negative in Mouse Lung, Rat Lung, Mouse Brain, Rat Brain and Mouse Skeletal Muscle using Anti-KHK Polyclonal Antibody (Product # PA5-29004) in Western Blot (Ref :DOI: 10.12688/f1000research.17082.2) {RE}

产品信息

PA5-29004

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Pig, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Full length human Ketohexokinase Recombinant protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.4 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2546480

产品详细信息

Recommended positive controls: HeLa, HepG2, mouse Liver.

Predicted reactivity: Mouse (87%), Rat (86%), Dog (85%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

KHK encodes the gene ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The splice variant presented encodes the highly active form found in liver. renal cortex. and small intestine. while the alternate variant encodes the lowe

仅用于科研。不用于诊断过程。未经明确授权不得转售。