Bethyl Laboratories

KIF14 Polyclonal Antibody

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KIF14 Antibody (A300-232A) in WB

Detection of human KIF14 by western blot and immunoprecipitation. Samples: Whole cell lysate (WCL; 50 µg for input; 1 mg/IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KIF14 antibodies BL357 (Cat. No. A300-232A) used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate. KIF14 was also immunoprecipitated using rabbit anti-KIF14 antibody BL358 (Cat. No. A300-233A). Detection: Chemiluminescence with an exposure time of 10 seconds.

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产品信息

A300-232A

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:10,000

免疫沉淀 (IP)

1-4 µg/mg lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residues 50 and 100 of human Kinesin Family Member 14.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

phosphate/tris citrate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

靶标信息

This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。