Invitrogen

KLHL3 Polyclonal Antibody

KLHL3 Antibody in Immunocytochemistry (ICC/IF)

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KLHL3 Antibody (PA5-118894) in ICC/IF

Immunocytochemical analysis of KLHL3 in A549 cells using a KLHL3 polyclonal antibody (Product # PA5-118894). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with the primary antibody and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).

KLHL3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-118894

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human KLHL3(Accession Q9UH77), corresponding to amino acid residues M1-R44.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2903394

产品详细信息

Antibody detects endogenous levels of total KLHL3.

靶标信息

Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。