Invitrogen

KMT2D Polyclonal Antibody

KMT2D Antibody in Immunocytochemistry (ICC/IF)

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KMT2D Antibody (PA5-49581) in ICC/IF

Immunofluorescence analysis of Histone-lysine N-methyltransferase 2D was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with KMT2D Polyclonal Antibody (Product # PA5-49581) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Ale... View More

KMT2D Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-49581

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:27,000

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免疫组化（石蜡） (IHC (P))

1:500

免疫细胞化学 (ICC/IF)

1:100

功能检测 (Functional)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 4980-5009 amino acids from the C-terminal region of human MLL2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2635035

靶标信息

KMT2D is a histone methyltransferase that methylates Lys-4 of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. KMT2D acts as a coactivator for estrogen receptors by being recruited by ESR1 and activating transcription. KMT2D is involved in chromatin organization, chormatin silencig, oocyte growth, oogenesis, and a positive regulation of cell proliferation. Mutations in the KMT2D gene cause Kabuki syndrome (KABUK1) which results in congential mental retardation syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.

The Journal of clinical investigation

Zhang Y,Yu H,Zhang J,Gao H,Wang S,Li S,Wei P,Liang J,Yu G,Wang X,Li X,Li D,Yang W

PA5-49581 was used in Functional Assay to reveal a potentially new role and mechanism of SAM-promoted tumor metastasis but also demonstrate a regulatory mechanism of PHGDH activity by monoubiquitination.

Mon Nov 01 00:00:00 EDT 2021

The low-complexity domains of the KMT2D protein regulate histone monomethylation transcription to facilitate pancreatic cancer progression.

Cellular & molecular biology letters

Li W,Wu L,Jia H,Lin Z,Zhong R,Li Y,Jiang C,Liu S,Zhou X,Zhang E

Published figure using KMT2D polyclonal antibody (Product # PA5-49581) in Western Blot

Wed Nov 10 00:00:00 EST 2021

生物信息学

蛋白别名: ALL1-related protein; Histone-lysine N-methyltransferase 2D; histone-lysine N-methyltransferase MLL2; Kabuki make-up syndrome; Kabuki mental retardation syndrome; lysine (K)-specific methyltransferase 2D; Lysine N-methyltransferase 2D; myeloid/lymphoid or mixed-lineage leukemia 2; Myeloid/lymphoid or mixed-lineage leukemia protein 2; trinucleotide repeat containing 21

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基因别名: AAD10; ALR; CAGL114; KABUK1; KMS; KMT2D; MLL2; MLL4; TNRC21

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UniProt ID: (Human) O14686

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Entrez Gene ID: (Human) 8085

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功能

DNA binding protein binding zinc ion binding histone-lysine N-methyltransferase activity histone methyltransferase activity (H3-K4 specific) transcription regulatory region DNA binding

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