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KREMEN2 Antibody (PA5-34525) in ICC/IF
Immunocytochemistry of HeLa cells using KREMEN2 Polyclonal Antibody (Product # PA5-34525) at 5 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
产品信息
PA5-34525
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A peptide corresponding to 14 amino acids near the carboxy terminus of human KREMEN2. The immunogen is located within amino acids 390-440 of KREMEN2.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
0.02% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2551877
产品详细信息
A suggested positive control is Hela cell lysate.
PA5-34525 can be used with blocking peptide PEP-1568.
靶标信息
Kremen (Kringle containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs. Kremens bind a subset of the secreted Dickkopf proteins (Dkk 1, 2, and 4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, Frizzled, and the LDL receptor related protein 5/6 (LRP5/6) co-receptor. Recent experiments have shown that KREMEN2 is a regulator of bone remodeling and raise the possibility that antagonizing KREMEN2 might prove beneficial in patients with bone loss disorders.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Dickkopf receptor 2; Kremen protein 2; Kringle domain-containing transmembrane protein 2; Kringle-containing protein marking the eye and the nose
基因别名: 2900054E04Rik; KREMEN2; KRM2
UniProt ID: (Human) Q8NCW0, (Mouse) Q8K1S7
Entrez Gene ID: (Human) 79412, (Mouse) 73016
