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Detection of KT3-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-KT3 antibody A190-129A used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
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产品信息
A190-129A
应用
建议稀释比
已发表文章
免疫印迹
(WB)
1:1,000 - 1:30,000
-
免疫细胞化学
(ICC/IF)
1:100 - 1:400
-
酶联免疫吸附实验
(ELISA)
1:1,000 - 1:30,000
-
免疫沉淀
(IP)
1 - 4 µg/mg lysate
-
产品规格
种属反应
Tag
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 6.8 to 7.4
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Wet ice
产品详细信息
Goats were immunized with KT3 (KPPTPPPEPET) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.