Invitrogen

Kir2.1 (KCNJ2) Monoclonal Antibody (S112), FITC

Kir2.1 (KCNJ2) Antibody in Immunocytochemistry (ICC/IF)

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Kir2.1 (KCNJ2) Antibody (MA5-45416) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Kir2.1 monoclonal antibody (Product # MA5-45416) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM f... View More

Kir2.1 (KCNJ2) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Kir2.1 (KCNJ2) Antibody in Western Blot (WB)

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产品信息

MA5-45416

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:1,000

免疫细胞化学 (ICC/IF)

1:100

Antibody Microarray (AM)

Assay-dependent

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

S112

抗原

Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1

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偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine

内含物

no preservative

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2931870

产品详细信息

1 µg/mL of MA5-45416 was sufficient for detection of Kir2.1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 45kDa. No cross-reactivity against Kir2.2 or Kir2.3.

靶标信息

The KIR2 subunit family includes 2. 1, 2. 2, 2. 3 and 2. 4. Unlike G protein-coupled KIR3 subunits, KIR2. 1 requires both phosphorylation by PKA and ATP hydrolysis for functional activity. KIR2. 1 is expressed in the superior and inferior collicula and the pontine region of the brain, where it moderates synaptic transmission, like many other potassium channels. In the placenta, KIR2. 1 is expressed throughout gestation in cytotrophoblast cells. In the kidney, KIR2. 1 colocalizes with KIR5. 1 in the proximal tubule. KIR2. 1, 2. 2 and 2. 3 associate with the membrane-associated guanylate kinase synapseassociated protein 97 in the cerebellum and heart. Phosphorylation of KIR2. 2 by protein kinase A inhibits the associates with SAP97.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Cardiac inward rectifier potassium channel; HHIRK 1; HIRK 1; hIRK1; Inward rectifier K; Inward rectifier K(+) channel Kir2.1; inward rectifier K+ channel KIR2.1; Inward rectifier potassium channel 2; IRK 1; IRK-1; IRK2; LQT 7; Potassium channel, inwardly rectifying subfamily J member 2; potassium channel, inwardly rectifying subfamily J, member 2; potassium inwardly-rectifying channel, subfamily J, member 2; RBL-IRK1; SQT 3

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基因别名: ATFB9; HHBIRK1; HHIRK1; IRK1; Kcnf1; KCNJ2; KIR2.1; LQT7; SQT3

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UniProt ID: (Human) P63252, (Rat) Q64273, (Mouse) P35561

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Entrez Gene ID: (Human) 3759, (Rat) 29712, (Mouse) 16518

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功能

inward rectifier potassium channel activity phosphatidylinositol-4,5-bisphosphate binding G-protein activated inward rectifier potassium channel activity identical protein binding voltage-gated potassium channel activity involved in cardiac muscle cell action potential repolarization voltage-gated ion channel activity protein binding ion channel transporter

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