Abnova

LARP1 Polyclonal Antibody, MaxPab™

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LARP1 Antibody (H00023367-B01P) in WB

Western Blot analysis of LARP1 expression in transfected 293T cell line by LARP1 MaxPab polyclonal antibody. Lane 1: LARP1 transfected lysate(112.09 KDa). Lane 2: Non-transfected lysate. .

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产品信息

H00023367-B01P

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG

分类

Polyclonal

类型

Antibody

抗原

LARP1 (NP_056130, 1 a.a. approximately 1019 a.a) full-length human protein.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Product may be used with Western Blot (Transfected lysate).

The Isotype of this product is composed of an IgG Mixture.

Immunogen sequence: MLWRVLLSKR PPFPHPELDF QEAPIPSCPG RLPGRKNSVA LAAAPRKEPT GDREKPLPFP VLAPFSNPEH SAPAKVVRAA VPKQRKGSKV GDFGDAINWP TPGEIAHKSV QPQSHKPQPT RKLPPKKDMK EQEKGEGSDS KESPKTKSDE SGEEKNGDED CQRGGQKKKG NKHKWVPLQI DMKPEVPREK LASRPTRPPE PRHIPANRGE IKGSESATYV PVAPPTPAWQ PEIKPEPAWH DQDETSSVKS DGAGGARASF RGRGRGRGRG RGRGRGGTRT HFDYQFGYRK FDGVEGPRTP KYMNNITYYF DNVSSTELYS VDQELLKDYI KRQIEYYFSV DNLERDFFLR RKMDADGFLP ITLIASFHRV QALTTDISLI FAALKDSKVV EIVDEKVRRR EEPEKWPLPP IVDYSQTDFS QLLNCPEFVP RQHYQKETES APGSPRAVTP VPTKTEEVSN LKTLPKGLSA SLPDLDSENW IEVKKRPRPS PARPKKSEES RFSHLTSLPQ QLPSQQLMSK DQDEQEELDF LFDEEMEQMD GRKNTFTAWS DEESDYEIDD RDVNKILIVT QTPHYMRRHP GGDRTGNHTS RAKMSAELAK VINDGLFYYE QDLWAEKFEP EYSQIKQEVE NFKKVNMISR EQFDTLTPEP PVDPNQEVPP GPPRFQQVPT DALANKLFGA PEPSTIARSL PTTVPESPNY RNTRTPRTPR TPQLKDSSQT SRFYPVVKEG RTLDAKMPRK RKTRHSSNPP LESHVGWVMD SREHRPRTAS ISSSPSEGTP TVGSYGCTPQ SLPKFQHPSH ELLKENGFTQ HVYHKYRRRC LNERKRLGIG QSQEMNTLFR FWSFFLRDHF NKKMYEEFKQ LALEDAKEGY RYGLECLFRY YSYGLEKKFR LDIFKDFQEE TVKDYEAGQL YGLEKFWAFL KYSKAKNLDI DPKLQEYLGK FRRLEDFRVD PPMGEEGNHK RHSVVAGGGG GEGRKRCPSQ SSSRPAAMIS QPPTPPTGQP VREDAKWTSQ HSNTQTLGK

靶标信息

RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability (PubMed:20430826, PubMed:23711370, PubMed:24532714, PubMed:25940091, PubMed:28650797, PubMed:28673543, PubMed:29244122). Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery (PubMed:23711370, PubMed:25940091, PubMed:28650797, PubMed:29244122, PubMed:26206669, PubMed:28379136). The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1 (PubMed:25940091, PubMed:28650797, PubMed:29244122, PubMed:28379136). When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA (PubMed:25940091, PubMed:28650797). Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif (PubMed:28379136). Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes (PubMed:20430826, PubMed:24532714, PubMed:25940091, PubMed:28673543). Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis (PubMed:23711370, PubMed:28650797). Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation (PubMed:20430826, PubMed:24532714). Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis (PubMed:20430826, PubMed:23711370, PubMed:25940091, PubMed:28673543). [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。