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LIGHT Antibody (500-P308BT-25UG) in WB
Western Blot: To detect Murine LIGHT by Western Blot analysis LIGHT Polyclonal Antibody, Biotin (Product # 500-P308BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine LIGHT is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P308BT-25UG
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine LIGHT
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930324
产品详细信息
AA Sequence of recombinant protein: MRLHQRLGDI VAHLPDGGKG SWEKLIQDQR SHQANPAAHL TGANASLIGI GGPLLWETRL GLAFLRGLTY HDGALVTMEP GYYYVYSKVQ LSGVGCPQGL ANGLPITHGL YKRTSRYPKE LELLVSRRSP CGRANSSRVW WDSSFLGGVV HLEAGEEVVV RVPGNRLVRP RDGTRSYFGA FMV.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine LIGHT. Anti-Murine LIGHT-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine LIGHT by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine LIGHT (500-P308) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Murine LIGHT.
Western Blot: To detect Murine LIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine LIGHT is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P308BT-1MG will be provided as 2 x 500 µg
靶标信息
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD258; sCD258; sLIGHT; soluble CD258; soluble LIGHT; Tumor necrosis factor ligand superfamily member 14
基因别名: HVEM-L; HVEML; LIGHT; LTg; Ly113; Tnfsf14
UniProt ID: (Mouse) Q9QYH9
Entrez Gene ID: (Mouse) 50930
