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LIN28A Antibody (MA1-016-D488) in ICC/IF
Immunofluorescent analysis of Lin28 (green) showing cytoplasmic staining of NCCIT cells (right panel) compared to HeLa cells (left panel). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 488-conjugated Lin28 monoclonal antibody (Product # MA1-016-D488) in 3% BSA-PBS at a dilution of 1:100 and incubated for 1 hour at 37C in the dark. F-Actin (both panels, red) was stained with DyLight ... View More
产品信息
MA1-016-D488
产品规格
种属反应
Human,
Mouse
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
14E6-4E6
抗原
Full-length human recombinant protein expressed in bacteria
偶联物
激发/发射光谱
492/519 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with proprietary stabilizer
内含物
0.02% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536673
产品详细信息
MA1-016-D488 has been successfully used in ICC/IF and flow cytometry applications on human and mouse samples.
MA1-016-D488 can be used for immunofluorescence analysis of LIN28 in human iPSCs.
靶标信息
Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ12457; Lin-28A; Protein lin-28 homolog A; RNA-binding protein LIN-28; testis expressed gene 17; Testis-expressed protein 17; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1
基因别名: AL024421; CSDD1; ENSMUSG00000070700; Gm10299; LIN-28; lin-28A; LIN28; LIN28A; Tex17; ZCCHC1
UniProt ID: (Human) Q9H9Z2, (Mouse) Q8K3Y3
Entrez Gene ID: (Human) 79727, (Mouse) 83557
regulation of transcription, DNA-templated
germ cell development
miRNA catabolic process
stem cell population maintenance
pre-miRNA processing
RNA 3'-end processing
somatic stem cell population maintenance
positive regulation of neuron differentiation
negative regulation of glial cell differentiation
positive regulation of translation
stem cell differentiation
regulation of gene silencing by miRNA
positive regulation of cell proliferation involved in kidney development
miRNA metabolic process
gene silencing by RNA
