Invitrogen

LMAN1 Monoclonal Antibody (OTI1C5)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他14个LMAN1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (13)
高级验证 (1)

LMAN1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

LMAN1 Antibody (MA5-25373) in ICC/IF

Immunofluorescence analysis of LMAN1 was performed using 70% confluent log phase HeLa cells treated with Brefeldin A (5 µg/mL, 15 minutes). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with LMAN1 Monoclonal Antibody (OTI1C5) (Product # MA5-25373) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombina... View More

LMAN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

LMAN1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with LMAN1 siRNA and reduction of signal was observed in Western Blot using LMAN1 Monoclonal Antibody (OTI1C5) (Product # MA5-25373). {KD}

产品信息

MA5-25373

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:150

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1:100

产品规格

种属反应

Human, Non-human primate

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI1C5

抗原

Full length human recombinant protein of LMAN1 produced in HEK293T cell

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2724608

靶标信息

The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: endoplasmic reticulum-golgi intermediate compartment protein 53; ER-Golgi intermediate compartment 53 kDa protein; Gp58; Intracellular mannose-specific lectin MR60; Lectin mannose-binding 1; lectin, mannose-binding, 1; Protein ERGIC-53

查看更多收起

基因别名: ERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; LMAN1; MCFD1; MR60

查看更多收起

UniProt ID: (Human) P49257

查看更多收起

Entrez Gene ID: (Human) 3998

查看更多收起

功能

protein binding mannose binding metal ion binding unfolded protein binding membrane traffic protein

参与通路

protein folding ER to Golgi vesicle-mediated transport endoplasmic reticulum organization Golgi organization blood coagulation positive regulation of organelle organization protein N-linked glycosylation via asparagine protein exit from endoplasmic reticulum early endosome to Golgi transport COPII vesicle coating