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Invitrogen

LXR alpha Polyclonal Antibody

2个已发表图片

8 篇参考文献

查看其他14个LXR alpha抗体

说明书

实验方案

技术支持

货号 PA1-330

CNY 4,717.00

200 µg

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说明书

实验方案

技术支持

抗体检测数据 (3)
已发表图片 (2)

Application

LXR alpha Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

LXR alpha Antibody (PA1-330) in ICC/IF

Immunofluorescent analysis of LXR alpha (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a LXR alpha polyclonal antibody (Product # PA1-330) in 3% BSA-PBS at ... View More

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产品信息

PA1-330

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:500

查看1篇已发表文章 1篇已发表文章

凝胶阻滞分析 (GS)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues C D(318) F S Y N R E D F A K A(329) of human LXR alpha.

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_325600

产品详细信息

PA1-330 detects recombinant human liver X receptor (LXR) alpha. This antibody does not detect human recombinant LXR beta protein.

PA1-330 has been successfully used in ICC/IF and Western blot procedures. By Western blot, this antibody detects an ~64 kDa protein representing recombinant human LXR alpha.

The PA1-330 immunogen is a synthetic peptide corresponding to residues C D(318) F S Y N R E D F A K A(329) of human LXR alpha. The PA1-330 immunizing peptide (Cat. # PEP-114) is available for use in neutralization and control experiments.

靶标信息

NR1H3 (oxysterols receptor LXR-alpha) is a nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Its interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanucleotide half-sites spaced by four nucleotides. NR1H3 plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR, and LRP8. It also interplays functionally with RORA for the regulation of genes involves in liver metabolism.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

LXRα Regulates oxLDL-Induced Trained Immunity in Macrophages.

International journal of molecular sciences

Findeisen HM,Voges VC,Braun LC,Sonnenberg J,Schwarz D,Körner H,Reinecke H,Sohrabi Y

PA1-330 was used in Western Blotting to reveal important aspects of LXR signaling in innate immunity with relevance to atherosclerosis formation.

Tue May 31 00:00:00 UTC 2022

Sectm1a deficiency aggravates inflammation-triggered cardiac dysfunction through disruption of LXRα signalling in macrophages.

Cardiovascular research

Li Y,Deng S,Wang X,Huang W,Chen J,Robbins N,Mu X,Essandoh K,Peng T,Jegga AG,Rubinstein J,Adams DE,Wang Y,Peng J,Fan GC

PA1-330 was used in Immunohistochemistry (PFA fixed) to define Sectm1a as a new regulator of inflammatory-induced cardiac dysfunction through modulation of LXRα signalling in macrophages.

Mon Feb 22 00:00:00 UTC 2021

Administration of GDF3 Into Septic Mice Improves Survival via Enhancing LXRα-Mediated Macrophage Phagocytosis.

Frontiers in immunology

Wang P,Mu X,Zhao H,Li Y,Wang L,Wolfe V,Cui SN,Wang X,Peng T,Zingarelli B,Wang C,Fan GC

PA1-330 was used in Immunocytochemistry-immunoflourescence to show that administration of recombinant GDF3 protein (rGDF3) either before or after CLP surgery improved mouse survival, along with significant reductions in bacterial load, plasma pro-inflammatory cytokine levels, and organ damage.

Mon Sep 27 00:00:00 UTC 2021

Administration of GDF3 Into Septic Mice Improves Survival via Enhancing LXRα-Mediated Macrophage Phagocytosis.

Frontiers in immunology

Wang P,Mu X,Zhao H,Li Y,Wang L,Wolfe V,Cui SN,Wang X,Peng T,Zingarelli B,Wang C,Fan GC

Mon Sep 27 00:00:00 UTC 2021

Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis.

Molecular therapy : the journal of the American Society of Gene Therapy

Xu Y,Xu Y,Zhu Y,Sun H,Juguilon C,Li F,Fan D,Yin L,Zhang Y

PA1-330 was used in Western Blotting to investigate the mechanism of microRNA-34a (miR-34a) that regulating macrophage cholesterol efflux and reverse cholesterol transport.

Wed Jan 08 00:00:00 UTC 2020

27-hydroxycholesterol decreases cell proliferation in colon cancer cell lines.

Biochimie

Warns J,Marwarha G,Freking N,Ghribi O

Published figure using LXR alpha polyclonal antibody (Product # PA1-330) in Western Blot

Mon Oct 01 00:00:00 UTC 2018

Liver X receptor is a therapeutic target in collagen-induced arthritis.

Arthritis and rheumatism

Chintalacharuvu SR,Sandusky GE,Burris TP,Burmer GC,Nagpal S

PA1-330 was used in immunohistochemistry to demonstrate that liver X receptor inhibits the progression of arthritis symptoms in collagen-induced arthritis

Sun Apr 01 00:00:00 UTC 2007

Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: differential role of LXR.

Journal of lipid research

Khovidhunkit W,Moser AH,Shigenaga JK,Grunfeld C,Feingold KR

PA1-330 was used in western blot to investigate the role of liver X receptor (LXR) in the regulation of ATP binding cassette (ABC) transporters

Mon Sep 01 00:00:00 UTC 2003

Oxysterol-activated LXRalpha/RXR induces hSR-BI-promoter activity in hepatoma cells and preadipocytes.

Biochemical and biophysical research communications

Malerød L,Juvet LK,Hanssen-Bauer A,Eskild W,Berg T

PA1-330 was used in EMSA to investigate the regulatory effect of LXR on SR-BI expression in hepatoma cells and preadipocytes

Fri Dec 20 00:00:00 UTC 2002

生物信息学

蛋白别名: liver X nuclear receptor alpha variant 1; Liver X receptor alpha; LXRb; NER; NERI; NR1H2; Nuclear receptor subfamily 1 group H member 3; nuclear receptor subfamily 1, group H, member 3; Oxysterols receptor LXR-alpha

基因别名: LXR-a; LXRA; NR1H3; RLD-1

UniProt ID: (Human) Q13133

Entrez Gene ID: (Human) 10062

功能

DNA binding steroid hormone receptor activity transcription coactivator activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding zinc ion binding cholesterol binding sterol response element binding sequence-specific DNA binding transcription regulatory region DNA binding

参与通路

LXR alpha Polyclonal Antibody

LXR alpha Antibody (PA1-330) in ICC/IF

产品信息

PA1-330

免疫印迹 (WB)

免疫组化 (IHC)

免疫组织化学（多聚甲醛固定） (IHC (PFA))

免疫细胞化学 (ICC/IF)

凝胶阻滞分析 (GS)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

LXR alpha Polyclonal Antibody

LXR alpha Antibody (PA1-330) in ICC/IF

产品信息

PA1-330

免疫印迹 (WB)

免疫组化 (IHC)

免疫组织化学（多聚甲醛固定） (IHC (PFA))

免疫细胞化学 (ICC/IF)

凝胶阻滞分析 (GS)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID