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Lamin-A Antibody (A303-432A) in ICC/IF
Detection of human Lamin-A by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- Lamin-A (Cat. No. A303-432A Lot1) used at a dilution of 1:200 (1 µg/mL). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.
产品信息
A303-432A
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 400 and 450 of human Lamin A.
偶联物
形式
Liquid
浓度
0.20 mg/mL
纯化类型
Antigen affinity chromatography
保存液
TBS, pH 7.0 to 8.0, with 0.1% BSA
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Wet ice
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For ICC, formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Rat and Pig
靶标信息
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 70 kDa lamin; lamin A/C-like 1; lamin C; lamin-A/C; OTTHUMP00000015843; OTTHUMP00000015846; OTTHUMP00000015847; Prelamin-A/C; renal carcinoma antigen NY-REN-32
基因别名: CDCD1; CDDC; CMD1A; CMT2B1; Dhe; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; PRO1
UniProt ID: (Human) P02545, (Mouse) P48678
Entrez Gene ID: (Human) 4000, (Mouse) 16905
nucleus organization
nuclear envelope organization
muscle organ development
establishment of cell polarity
regulation of cell migration
establishment or maintenance of microtubule cytoskeleton polarity
protein localization to nucleus
sterol regulatory element binding protein import into nucleus
ventricular cardiac muscle cell development
negative regulation of release of cytochrome c from mitochondria
positive regulation of cell aging
regulation of protein localization to nucleus
negative regulation of extrinsic apoptotic signaling pathway
mitotic nuclear envelope disassembly
mitotic nuclear envelope reassembly
spermatogenesis
response to mechanical stimulus
IRE1-mediated unfolded protein response
positive regulation of osteoblast differentiation
cellular response to hypoxia
negative regulation of adipose tissue development
