Invitrogen

Lamin A Recombinant Rabbit Monoclonal Antibody (4H7)

Lamin A Antibody in Immunocytochemistry (ICC/IF)

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Lamin A Antibody (MA5-49796) in ICC/IF

Immunocytochemistry analysis of HeLa cells with Lamin A monoclonal antibody (Product # MA5-49796) at 1:38, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Lamin A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Lamin A Antibody in Flow Cytometry (Flow)

Lamin A Antibody in Immunoprecipitation (IP)

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产品信息

MA5-49796

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:20-1:200

流式细胞分析 (Flow)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

1:200-1:1,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

4H7

抗原

A synthesized peptide derived from human LMNA

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.6 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

RRID

AB_3092604

靶标信息

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.

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