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Invitrogen
TCR V alpha 2 Monoclonal Antibody (B20.1), NovaFluor™ Blue 610-70S, eBioscience™
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TCR V alpha 2 Antibody (M033T03B06-A) in Flow
C57BL/6 mouse splenocytes were unstained (left) or stained with 0.2 µg of TCR V alpha 2 Monoclonal Antibody, NovaFluor Blue 610-70S (right). All cells were co-stained with CD4 Monoclonal Antibody, eFluor 450 (Product # 48-0041-82) and CD8a Monoclonal Antibody, eFluor 450 (Product # 48-0081-82). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
产品信息
M033T03B06-A
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2a, lambda
分类
Monoclonal
类型
Antibody
克隆号
B20.1
偶联物
激发/发射光谱
492/616 nm
查看光谱
形式
Liquid
浓度
0.1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
RRID
AB_3099095 (AB_2926220)
产品详细信息
Description: The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. This antibody recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b and c haplotypes. The antibody also reacts with the products of T cell receptor, V delta 8 due to the high degree of homology.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.
Applications Reported: This B20.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This B20.1 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to preblock Fc receptors for 10 minutes using 0.5 µg of purified anti-mouse CD16/32 (Product # 14-0161-82). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Product # C001T06F01). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 509 nm; Emission: 614 nm; Laser: 488 nm (Blue) Laser
.
靶标信息
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: TCRVA; TCRVA2; V alpha 2; V alpha-2 TCR; Va 2; Va2; Valpha 2; Valpha2
