Invitrogen

Clathrin Heavy Chain Monoclonal Antibody (X22), Alexa Fluor™ 488

Clathrin Heavy Chain Antibody in Immunocytochemistry (ICC/IF)

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Clathrin Heavy Chain Antibody (MA1-065-A488) in ICC/IF

Immunofluorescent analysis of Clathrin Heavy Chain (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Clathrin Heavy Chain Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA1-065-A488) at a dilution of 5 µg/mL in blocking buffer ... View More

Clathrin Heavy Chain Antibody in Immunocytochemistry, Immunoprecipitation (ICC/IF, IP)

Clathrin Heavy Chain Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

Clathrin Heavy Chain Antibody in Western Blot (WB)

Clathrin Heavy Chain Antibody in Immunoprecipitation (IP)

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产品信息

MA1-065-A488

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看4篇已发表文章 4篇已发表文章

免疫细胞化学 (ICC/IF)

1-10 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫沉淀 (IP)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

X22

抗原

Purified human brain clathrin heavy chain

查看免疫原

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2662916

产品详细信息

MA1065A488 detects clathrin heavy chain in non-human primate, bovine, human, rat and mouse tissues as well as hamster (CHO) cells.

The MA1065A488 immunogen is purified human brain clathrin heavy chain. Electron microscopy and proteolysis mapping demonstrate that MA1065A488 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.

靶标信息

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients.

Frontiers in molecular neuroscience

Poulsen ET,Iannuzzi F,Rasmussen HF,Maier TJ,Enghild JJ,Jørgensen AL,Matrone C

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Immunoprecipitation

Mon Sep 25 00:00:00 EDT 2023

An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients.

Frontiers in molecular neuroscience

Poulsen ET,Iannuzzi F,Rasmussen HF,Maier TJ,Enghild JJ,Jørgensen AL,Matrone C

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Immunoprecipitation

Mon Sep 25 00:00:00 EDT 2023

ROCK1 is a novel Rac1 effector to regulate tubular endocytic membrane formation during clathrin-independent endocytosis.

Scientific reports

Soriano-Castell D,Chavero A,Rentero C,Bosch M,Vidal-Quadras M,Pol A,Enrich C,Tebar F

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Western Blot

Mon Jul 31 00:00:00 EDT 2017

Surface Toll-like receptor 3 expression in metastatic intestinal epithelial cells induces inflammatory cytokine production and promotes invasiveness.

The Journal of biological chemistry

Bugge M,Bergstrom B,Eide OK,Solli H,Kjønstad IF,Stenvik J,Espevik T,Nilsen NJ

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Western Blot

Fri Sep 15 00:00:00 EDT 2017

Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells.

PloS one

Chiang CF,Flint M,Lin JS,Spiropoulou CF

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Western Blot

Tue Jun 13 00:00:00 EDT 2017

Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance.

Nature neuroscience

Zhao Z,Sagare AP,Ma Q,Halliday MR,Kong P,Kisler K,Winkler EA,Ramanathan A,Kanekiyo T,Bu G,Owens NC,Rege SV,Si G,Ahuja A,Zhu D,Miller CA,Schneider JA,Maeda M,Maeda T,Sugawara T,Ichida JK,Zlokovic BV

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Immunofluorescence

Wed Jul 01 00:00:00 EDT 2015

Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance.

Nature neuroscience

Zhao Z,Sagare AP,Ma Q,Halliday MR,Kong P,Kisler K,Winkler EA,Ramanathan A,Kanekiyo T,Bu G,Owens NC,Rege SV,Si G,Ahuja A,Zhu D,Miller CA,Schneider JA,Maeda M,Maeda T,Sugawara T,Ichida JK,Zlokovic BV

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Immunofluorescence

Wed Jul 01 00:00:00 EDT 2015

Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance.

Nature neuroscience

Zhao Z,Sagare AP,Ma Q,Halliday MR,Kong P,Kisler K,Winkler EA,Ramanathan A,Kanekiyo T,Bu G,Owens NC,Rege SV,Si G,Ahuja A,Zhu D,Miller CA,Schneider JA,Maeda M,Maeda T,Sugawara T,Ichida JK,Zlokovic BV

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Immunofluorescence

Wed Jul 01 00:00:00 EDT 2015

The X-linked mental retardation protein OPHN1 interacts with Homer1b/c to control spine endocytic zone positioning and expression of synaptic potentiation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Nakano-Kobayashi A,Tai Y,Nadif Kasri N,Van Aelst L

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Immunofluorescence

Wed Jun 25 00:00:00 EDT 2014

生物信息学

蛋白别名: Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain on chromosome 17; Clathrin heavy chain on chromosome 22; clathrin, heavy chain (Hc); clathrin, heavy chain-like 1; clathrin, heavy polypeptide (Hc); Clathrin, heavy polypeptide D; clathrin, heavy polypeptide-like 1; clathrin, heavy polypeptide-like 2; CLH-17; CLH-22

查看更多收起

基因别名: 3110065L21Rik; CHC; CHC17; CHC22; CLH-17; CLH17; CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034; R74732

查看更多收起

功能

double-stranded RNA binding structural molecule activity protein binding protein kinase binding clathrin light chain binding poly(A) RNA binding protein domain specific binding identical protein binding disordered domain specific binding arrestin family protein binding signal transducer activity protein C-terminus binding ankyrin binding heat shock protein binding peptide binding vesicle coat protein

参与通路

osteoblast differentiation intracellular protein transport receptor-mediated endocytosis mitotic nuclear division antigen processing and presentation of exogenous peptide antigen via MHC class II receptor internalization transferrin transport retrograde transport, endosome to Golgi Wnt signaling pathway, planar cell polarity pathway negative regulation of hyaluronan biosynthetic process negative regulation of protein localization to plasma membrane autophagy clathrin coat assembly cell division signal transduction anatomical structure morphogenesis positive regulation of glucose import Golgi organization vesicle-mediated transport mitotic spindle assembly

Clathrin Heavy Chain Monoclonal Antibody (X22), Alexa Fluor™ 488

Clathrin Heavy Chain Antibody (MA1-065-A488) in ICC/IF

产品信息

MA1-065-A488

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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