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Invitrogen
TAP Tag Monoclonal Antibody (22F12-1E3), DyLight™ 680
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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TAP Tag Antibody (MA1-108-D680) in WB
Western blot was performed using Anti-TAP Tag Monoclonal Antibody (22F12-1E3), DyLight 680 (Product # MA1-108-D680) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), H3-TAP, 50 µg (Lane 3), H3-TAP, 25 µg (Lane 4), H3-TAP, 12.5 µg (Lane 5), H3-TAP, 6.25 µg (Lane 6), p65-TAP, 50 µg (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellu... View More
产品信息
MA1-108-D680
产品规格
种属反应
Tag
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
22F12-1E3
抗原
KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage
偶联物
激发/发射光谱
676/705 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536817
产品详细信息
MA1108-D680 detects the Tev cleavage peptide sequence. MA1108-D680 has been successfully used for fluorescent detection of TAP in Western Blot.
DyLight 680 has an excitation/emission of 692/712nm.
靶标信息
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "TAP tag" (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Tobacco Etch Virus
