Invitrogen

Nestin Monoclonal Antibody (10C2), Alexa Fluor™ 647

Nestin Antibody in Immunocytochemistry (ICC/IF)

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Nestin Antibody (MA1-110-A647) in ICC/IF

Immunofluorescent analysis of Nestin (red) in U2OS cells. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were stained with a Nestin mouse monoclonal antibody (Product # MA1-110-A647) at a concentration of 20 µg/mL in blocking buffer for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific Evos FL Auto Imaging system at 20X magni... View More

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产品信息

MA1-110-A647

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

10C2

抗原

Human nestin protein corresponding to residues 1464-1614

查看免疫原

偶联物

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

激发/发射光谱

650/671 nm 查看光谱

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A

保存液

PBS with 1.0mg/mL BSA

内含物

0.05% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Dry ice (international)

RRID

AB_2723401

产品详细信息

MA1-110 has been successfully utilized in Western Blot, immunocytochemistry, flow cytometry, and immunofluorescence applications in human samples.

靶标信息

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

仅用于科研。不用于诊断过程。未经明确授权不得转售。