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Invitrogen
6x-His Tag Monoclonal Antibody (4E3D10H2/E3), Alexa Fluor™ 647
-A647-Antibody-MA1-135-A647-IF-120160323131146.jpg?time=20220826 "6x-His Tag Antibody in Immunocytochemistry (ICC/IF)")
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6x-His Tag Antibody (MA1-135-A647) in ICC/IF
Immunofluorescent analysis of HeLa cells transfected with a construct containing a HIS Epitope Tag. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 inPBS for 15 minutes at room temperature and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with AlexaFluor 647 conjugated HIS-tag monoclonal antibody (Product # MA1-135-A647) ... View More
-A647-Antibody-MA1-135-A647-IF-120160323131146.jpg?time=20220826)
-A647-Antibody-MA1-135-A647-IF-120160323131146.jpg?time=20220826?time=20220826 "6x-His Tag Antibody (MA1-135-A647) in ICC/IF")
产品信息
MA1-135-A647
产品规格
种属反应
Tag
已发表种属
Tag
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
4E3D10H2/E3
抗原
Mixture of N-terminal and C-terminal 6x-His peptides, CG-HHHHHH and HHHHHH-GC
偶联物
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2610637
靶标信息
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 6His tag; 6X His; His Tag; Histidine Tag
