Invitrogen

CD14 Monoclonal Antibody (TüK4), FITC

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CD14 Antibody (MA1-82074) in Flow

Communications biology 2021 - Fig. 1 Malarial antibodies induce merozoite-phagocytosis by neutrophils and monocytes. a Percentage of individual leukocytes in PBLs from Danish blood donors ( n = 10) was enumerated by flow cytometry. Horizontal lines represent median values. b From each donor, 6 x 10 4 PBLs were incubated with unopsonized merozoites (UOP), merozoites opsonized with a pool of nonimmune plasma (NIP), or with immune plasma (... View More

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产品信息

MA1-82074

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Neat-1:2

查看3篇已发表文章 3篇已发表文章

其他PubMed (Misc)

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Bovine, Dog, Camelid, Cynomolgus monkey, Cat, Goat, Human, Mink, Sheep, Pig, Rabbit

已发表种属

Dog, Human, Rhesus monkey

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

TüK4

抗原

Proprietary Immunogen.

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Protein A

保存液

PBS with 1% BSA

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_927590

产品详细信息

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti human CD14 antibody, clone TÜK4 recognizes the human CD14 cell surface antigen.

靶标信息

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

The Stromal Vascular Fraction from Canine Adipose Tissue Contains Mesenchymal Stromal Cell Subpopulations That Show Time-Dependent Adhesion to Cell Culture Plastic Vessels.

Animals : an open access journal from MDPI

Scattini G,Pellegrini M,Severi G,Cagiola M,Pascucci L

MA1-82074 was used in Flow cytometry/Cell sorting to investigate their basic properties to speculate on the possible existence of mesenchymal stromal cells subpopulations.

Mon Mar 27 00:00:00 EDT 2023

Neutrophils dominate in opsonic phagocytosis of P. falciparum blood-stage merozoites and protect against febrile malaria.

Communications biology

Garcia-Senosiain A,Kana IH,Singh S,Das MK,Dziegiel MH,Hertegonne S,Adu B,Theisen M

Published figure using CD14 monoclonal antibody (Product # MA1-82074) in Flow Cytometry

Thu Aug 19 00:00:00 EDT 2021

Expression of co-stimulatory molecules CD80 and CD86 is altered in CD14 + HLA-DR + monocytes from patients with Chagas disease following induction by Trypanosoma cruzi recombinant antigens.

Revista da Sociedade Brasileira de Medicina Tropical

Soares AK,Neves PA,Cavalcanti MD,Marinho SM,Oliveira W,Souza JR,Lorena VM,Gomes YM

MA182074 was used in flow cytometry to measure CD80 and CD86 expression on CD14+HLA-DR+ monocytes from patients with Chagas disease

Tue Dec 20 00:00:00 EST 2016

Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.

Journal of virology

Hertel L,Lacaille VG,Strobl H,Mellins ED,Mocarski ES

MA1-82074 was used in flow cytometry to report that CD34(+) progenitor cell-derived Langerhans cells-type DCs exhibit a differentiation state-dependent susceptibility to CMV infection.

Tue Jul 01 00:00:00 EDT 2003

Flow cytometry in the bone marrow staging of mature B-cell neoplasms.

Cytometry. Part B, Clinical cytometry

Stacchini A,Demurtas A,Godio L,Martini G,Antinoro V,Palestro G

MA1-82074 was used in flow cytometry to ask if flow cytometry can be used for the detection of bone marrow involvement in the different types of B-cell malignant neoplasms.

Tue Jul 01 00:00:00 EDT 2003

Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction.

Clinical and diagnostic laboratory immunology

Hajishengallis G,Martin M,Sojar HT,Sharma A,Schifferle RE,DeNardin E,Russell MW,Genco RJ

MA1-82074 was used in flow cytometry to determine of proinflammatory cytokine responses induced by certain bacterial protein adhesins are dependent on TLRs.

Fri Mar 01 00:00:00 EST 2002

Cytokine expression, natural killer cell activation, and phenotypic changes in lymphoid cells from rhesus macaques during acute infection with pathogenic simian immunodeficiency virus.

Journal of virology

Giavedoni LD,Velasquillo MC,Parodi LM,Hubbard GB,Hodara VL

MA1-82074 was used in flow cytometry to examine the innate and adaptive immune responses of rhesus macaques infected with SIVmac251.

Tue Feb 01 00:00:00 EST 2000

生物信息学

蛋白别名: CD 14; CD14; CD14 antigen; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

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基因别名: CD14

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UniProt ID: (Rabbit) Q28680, (Human) P08571, (Bovine) Q95122