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NALCN Antibody (MA5-27593) in ICC/IF
Immunofluorescent analysis of NALCN in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with NALCN monoclonal antibody (Product # MA5-27593) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) NALCN Antibody, D) Merged image. Magnification... View More
产品信息
MA5-27593
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
N187/7
抗原
Fusion protein amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2735285
产品详细信息
1 µg/mL of MA5-27593 was sufficient for detection of NALCN in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 200kDa.
This antibody was formerly sold as clone S187-7.
靶标信息
Nalcn is a voltage-independent, cation-nonselective channel which is permeable to sodium, potassium and calcium ions. Nalcn regulates the resting membrane potential and controls neuronal excitability (PubMed:17448995). Neuropeptides such as neurotensin and substance P (SP) stimulate the firing of action potentials by activating NALCN through a SRC family kinases-dependent pathway. In addition to its baseline activity, Nalcn activity is enhanced/modulated by several GPCRs, is required for normal respiratory rhythm and neonatal survival, is involved in systemic osmoregulation by controlling the serum sodium concentration. Nalcn is partly responsible for the substance P-induced depolarization and regulation of the intestinal pace-making activity in the interstitial cells of Cajal. Further, Nalcn plays a critical role in both maintenance of spontaneous firing of substantia nigra pars reticulata (SNr) neurons and physiological modulation of SNr neuron excitability. Diseases associated with NALCN include Congenital contractures of the limbs and face, Hypotonia, Developmental Delay and Hypotonia, Infantile Psychomotor Retardation And Characteristic Facies 1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: brain voltage-gated cation channel; CanIon; FLJ23913; FLJ44659; FLJ44764; four domain-type voltage-gated ion channel alpha-1 subunit; four repeat voltage-gated ion channel; MGC74524; Sodium leak channel NALCN; Sodium leak channel non-selective protein; sodium leak channel, non selective; voltage gated channel like 1; Voltage gated channel-like protein 1
基因别名: A530023G15Rik; AI849508; bA430M15.1; CanIon; CLIFAHDD; IHPRF; IHPRF1; INNFD; NALCN; VGCNL1
UniProt ID: (Human) Q8IZF0, (Mouse) Q8BXR5
Entrez Gene ID: (Human) 259232, (Mouse) 338370, (Rat) 266760
calcium ion transport
ion transmembrane transport
regulation of ion transmembrane transport
sodium ion transmembrane transport
regulation of resting membrane potential
calcium ion transmembrane transport
potassium ion transmembrane transport
membrane depolarization during action potential
transport
ion transport
sodium ion transport
transmembrane transport
calcium ion import
sperm motility
cation transmembrane transport
